P-26 藍藻Microcystis viridis (NIES-102)より得られたトロンビン阻害物質(ポスター発表の部)

概要

論文の詳細を見る
We have found that blue-green algae Microcystis spp. are a new and rich source of unique protease inhibitors, and in fact have reported the isolation and structure elucidation of several protease inhibitors from some strains of M. aeruginosa. Here we describe the isolation and structure elucidation of aeruginosins 102-A(1) and B(2) from M. viridis (NIES-102). Microcystis viridis (NIES-102) was obtained from the National Institute for Environmental Studies, Japan. The 80% methanol extract of lyophilized alga (207g) was subjected to solvent partition, ODS column chromatography followed by ODS HPLC to obtain 1 and 2. Aeruginosins 102-A(1) and B(2) separeted into three peaks each in the HPLC due to the tautomerization of Argal in the same manner as in leupeptin. Molecular formula of 1 was decided as C_<33>H_<44>N_6O_<11>S from HRFABMS (731.2704 [M-H]^- Δ-0.7mmu) and NMR data. Amino acid analysis of the acid hydrolyzate gave Tyr. ^1H-^1H COSY, HMQC and HMBC spectra showed the presence of Hpla sulfate (p-hydroxyphenyllactic acid sulfate), Tyr, Choi (2-carboxy-6-hydroxyoctahydroindole) and Argal (argininal). Interpretation of HMBC and NOESY spectra revealed the planar structure of 1 and 2. The absolute stereochemistries of Hpla sulfate, Tyr and Argal were determined to be D-, D- and L-form by the chiral GC or HPLC, respectively. The structure of 2 were identical to those of 1 except the absolute stereochemistry of Argal(D-form). The IC_<50> of 1 and 2 against thrombin were 0.04 and 0.1μg/mL, respectively.
天然有機化合物討論会の論文
1995-09-01

P-26 藍藻Microcystis viridis (NIES-102)より得られたトロンビン阻害物質(ポスター発表の部)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク