Isolation of Restriction-reduced Mutants from Streptomyces(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
Restriction-reduced mutants were isolated from Streptomyces rosa subsp. notoensis KA301 and S. tanashiensis strain Kala which produce the benzoisochromanequinone antibiotics nanaomycin and kalafungin, respectively. The mutants of S. rosa, which can be transformed with a multi-copy plasmid and in which the actinophage Pa16 can propagate, were selected. They were transformed with a single-copy plasmid propagated in S. lividans TK24, and with its modified plasmid propagated in the mutant at higher efficiency. The mutants of 5. tanashiensis were selected by their capability to be transformed with a multi-copy plasmid. The efficiency of transformation with a single-copy plasmid propagated in S. lividans TK24 was low, but was much increased by heating the protoplasts at 42℃ for 15min prior to the transformation. These mutants derived from both strains probably lack at least one of their restriction systems.
- 社団法人日本農芸化学会の論文
- 1990-10-23
著者
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Kakinuma S
Kitasato Univ. Tokyo Jpn
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Kakinuma Shizuko
School Of Pharmaceutical Sciences Kitasato University Research Center For Biological Function The Ki
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IKEDA Haruo
School of Pharmaceutical Sciences, Kitasato University, Research Center for Biological Function, The
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TANAKA Haruo
School of Pharmaceutical Sciences, Kitasato University, Research Center for Biological Function, The
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OMURA Satoshi
School of Pharmaceutical Sciences, Kitasato University, Research Center for Biological Function, The
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Ikeda H
School Of Pharmaceutical Sciences Kitasato University The Kitasato Institute
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Ikeda Haruo
School Of Pharmaceutical Sciences Kitasato University
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Omura Satoshi
Research Center For Biological Function The Kitasato Institute And School Of Pharmaceutical Sciences
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Tanaka Y
School Of Pharmaceutical Sciences Kitasato University The Kitasato Institute
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Ikeda Hiroyuki
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Tanaka H
School Of Pharmaceutical Sciences Kitasato University And Research Center For Biological Function Th
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Tanaka Haruo
School Of Pharmaceutical Sciences Kitasato University
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Omura Satoshi
School Of Pharmaceutical Sciences Kitasato University And Kitasato Institute
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Ikeda Hiroyuki
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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