Screening and Fermentation of Endo-α-N-Acetylgalactosaminidase S, a Mucin-Hydrolyzing Enzyme from Streptomyces Acting on the GalNAc-Ο-Ser(Thr)Linkage
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概要
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Soil microorgaisms were examined for their ability to grow on porcine gastric mucin as a sole source of carbon and energy. Streptomyces sp.OH-11242 thus selected was found to produce endo-α-N-acetylgalactos-aminidase(endo-GalNAc-ase S), together with several mucin-degrading glycosidases. Endo-GalNAc-ase S is a new enzyme capable of hydrolyzing the innermost GalNAc-Ο-Ser(Thr)linkage of the mucin molecule. Studies on the fermentation conditions necessary for its production revealed that the enzyme was induced by mucin but the induction was inhibited by glucose and other easily assimilable carbon sources, as well as by complex nitrogen sources. Addition of palmitate, λ-carrageenan, or crude mucin to a mucin-based production medium enhanced enzyme production and mycelial growth. When the initial mucin concentration of the production medium was increased, the maximum titer of endo-GalNAc-ase S produced in the culture both also increased, while the cultivation time giving the peak enzyme titer was prolonged. As a result of the studies, increased and reproducible production of endo-GalNAc-ase S was achieved, reaching 42 units/ml in a 100-ml culture and 31 units/ml in an 800-ml culture with 2 and 1.5% purified mucin, respectively, as the major carbon source.
- 社団法人日本生物工学会の論文
- 1998-04-25
著者
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Takada Y
Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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Omura Satoshi
Research Center For Biological Function The Kitasato Institute And School Of Pharmaceutical Sciences
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TANAKA Yoshitake
Research Center for Biological Function
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TAKAHASHI Yoko
Research Center for Biological Function, The Kitasato Institute
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SHINOSE Mayumi
Research Center for Biological Function, The Kitasato Institute and Graduate School of Pharmaceutica
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Iwase Hitoo
Biochemistry School Of Medicine Kitasato University
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Hotta K
Department Of Biochemistry And Medicine School Of Medicine And Nursing Kitasato University
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Hotta Kyoko
Biochemistry School Of Medicine Kitasato University
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Shinose Mayumi
Research Center For Biological Function The Kitasato Institute And Graduate School Of Pharmaceutical
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Taniguchi Y
Kitasato Institute For Life Sciences Kitasato University
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Tanaka Y
Research Center For Biological Function
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Shibata M
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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-KARAKASA IKUKO
Biochemistry, School of Medicine, Kitasato University
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Tanaka Y
Research Center For Biological Function The Kitasato Institute
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Shinose Mayumi
Research Center For Biological Function The Kitasato Institute
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Omura Satoshi
Research Center For Biological Function The Kitasato Institute
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Sugoh-hagino Masako
Research Center For Biological Function The Kitasato Institute
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