A Rapid and Simple Procedure for Purification of S-II, a Transcription Factor of Ribonucleic Acid Polymerase II(Biological)

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We previously reported the purification of eukaryotic transcription factor S-II and its phosphorylated form S-H' from Ehrlich ascites tumor cells. In this work, we modified the previous purification procedure to make it quicker and simpler. By this improved procedure, involving a different solubilization method, and improved buffer solutions and centrifugation processes, S-II could be purified to homogeneity from 1 kg of frozen cells in about 10 d instead of 1 month. The purity, specific activity and total yield of S-II purified by the new method were comparable to those obtained by using the previous method.
社団法人日本薬学会の論文
1987-02-25