歯槽骨吸収に及ぼすOsteoclast-activating factor (OAF)の効果 : 1. OAFの産生とその精製の試み

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We have characterized osteoclast-activating factor (OAF) using a bioassay for bone resorption which utilizes the release of ^<45>Ca from fetal rat calvaria in vitro. OAF was obtained from supernatants of cultures of either normal human leukocytes (PEL), or tonsil cells activated with phytohemagglutinin (PHA) or dental plaques from patients with periodontal disease. Furthermore, T and non-T cells were separated from PBL by means of sheep erythrocyte rosette formation, and each fraction was cultured with PHA. The results suggest that activated T cells can produce OAF, and its production is enhanced by plastic-adherent cells (monocyte). 100% of the OAF activity was retained on Amicon UM2 membranes with a nominal molecular weight cutoff of 1,000 daltons, and 60% of the activity was retained on Amicon PM 10 membranes with a nominal molecular weight cutoff of 10,000 daltons. These results indicate that the molecular weight of OAF is about 10,000 daltons.
特定非営利活動法人日本歯周病学会の論文
1982-03-28