Effect of Aralia cordata Extracts on Cartilage Protection and Apoptosis Inhibition(Pharmacognosy)

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概要

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• Cartilage loss in osteoarthritis is characterized by cartilage degradation and chondrocyte death. Cartilage degradation is induced by activation of matrix-metalloproteinases (MMPs) activity and degradation of glycosaminoglycan (GAG) and collagen. Also, chondrocyte death is induced by the apoptosis through the activation of MAP kinase and caspases activities. On the basis of this background, our study was designed to examine the cartilage protective and anti-apoptotic effect of Aralia cordata. Cartilage explants and Chondrocytes were cultured from rabbit knee joint cartilage and treated by 5ng/ml IL-1α. Cartilage and chondroprotective effects of Aralia cordata were determined by measuring (1) GAG and collagen expression, (2) GAG and collagen degradation, (3) TIMP and MMPs expression, and (4) TIMP and MMPs activity. Anti-apoptotic effects of Aralia cordata were determined by measuring (1) JNK and p38 MAP kinase expression, (2) apoptotic cells by flow cytometry, and (3) caspase-3 activity. In cartilage explants and chondroctyes treated by IL-1α, Aralia cordata showed the decrease of GAG and collagen degradation, decrease of MMPs (MMP-1, -3, -13) activity, and increase of TIMP-1 activity in a dose-dependent manner. Aralia cordata also showed anti-apoptotic effect by inhibition of early and late apoptotic cells, sub-G1 phase cells, and caspase-3 activity through the downregulation of JNK and p38 MAP kinase signaling pathway. Aralia cordata inhibited the cartilage and chondrocyte destruction through the down-regulation of MMPs activities and the inhibition of proteoglycan and collagen degradation. Also, Aralia cordata inhibited the chondrocyte apoptosis through the downregulation of JNK and p38 MAP kinase signal, and the inhibition of caspase-3 activity.

• 社団法人日本薬学会の論文
• 2006-07-01

著者

• Park Dong-suk

  Department Of Acupuncture & Moxibustion East-west Neo Medical Center Kyunghee University

• LEE Jae-Dong

  Department of Microbiology, College of Natural Sciences, Pusan National University

• Choi Do-young

  Department Of Acupuncture & Moxibustion College Of Oriental Medicine Kyunghee University

• Huh Jeong-eun

  Oriental Medicine Research Center For Bone And Joint Disease Kyunghee University

• BAEK Yong-Hyeon

  Department of Acupuncture & Moxibustion, East-West Neo Medical Center, KyungHee University

• Baek Yong-hyeon

  Department Of Acupuncture & Moxibustion East-west Neo Medical Center Kyunghee University

• Lee Jae-dong

  Department Of Acupuncture & Moxibustion College Of Oriental Medicine Kyunghee University

• Huh Jeong-eun

  Oriental Medicine Res. Center For Bone & Joint Disease Kyung Hee Univ.

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