A Lamination Micro Mixer for μ-Immunomagnetic Cell Sorter(<Special Issue>Bioengineering)
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概要
- 論文の詳細を見る
We report successful design, fabrication and testing of a novel lamination micro mixer to be integrated in the micro-scale immunomagnetic cell sorter (μ,-IMCS), which should be a key device for clinical applications of regenerative medicine. This paper covers, (i) the concept of μ-IMCS, (ii) design and fabrication of lamination mixer using MEMS technologies, (iii) numerical analysis for the evaluation of the mixer performance, and (iv) experimental evaluation of target cell capturing with the present micro mixer. In order to reduce the sedimentation loss of the cells and the magnetic beads, the conduits in the mixer are designed in such a way that the stream is turned over 180 degree. It is found in the CFD analysis that the present lamination mixer realizes better mixing and lower sedimentation loss than the one without rotation in the previous study. Experiments reveal that the cell capture rate is (i) increased by up to 8.6 times compared with that in a straight channel, and (ii) decreased with decreasing the residence time. It is also demonstrated in a preliminary experiment that CD31 expressions of HUVEC and hMSC obtained with the present micro mixer are in good agreement with the data obtained with a conventional cell sorting system. Therefore, the present mixer should be a viable component in a μ-IMCS.
- 一般社団法人日本機械学会の論文
- 2005-12-15
著者
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Ushida Takashi
Center For Disease Biology And Integrative Medicine The University Of Tokyo
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Furukawa Katsuko
Department Of Bioengineering The University Of Tokyo
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Furukawa Katsuko
Department Of Mechanical Engineering The University Of Tokyo
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Tan Wei-heong
Department Of Mechanical Engineering The University Of Tokyo:(present Address)cirmm Institute Of Ind
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Kasagi Nobuhide
Department Of Mechanical Engineering The University Of Tokyo
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Ushida Takashi
Center For Disease Biology And Integrative Medicine School Of Medicine The University Of Tokyo
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Suzuki Yuji
Department Of Public Health And Environmental Medicine Jikei University School Of Medicine
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SHIKAZONO Naoki
Department of Mechanical Engineering, The University of Tokyo
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Kasagi Nobuhide
Univ. Tokyo Tokyo Jpn
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Shikazono Naoki
Department Of Mechanical Engineering The University Of Tokyo
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Suzuki Yuji
Department Of Mechanical Engineering The University Of Tokyo
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Suzuki Yuji
Department Of Health Sciences School Of Health And Social Services Saitama Prefectural University
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Suzuki Yuji
Department Of Astronomy Kyoto University
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SUZUKI Yuji
Department of Agriculture, Graduate School of Agricultural Science, Tohoku University
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