The Effect of δ-Elemene on Hela Cell Lines by Apoptosis Induction
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概要
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This study was designed to investigate the apoptosis-inducing activity of δ-elemene on Hela cells in vitro. MTT assay and Hoechst 33258/PI fluorescence microscopy were used for this investigation. Apoptosis was further confirmed and quantified by DNA fragmentation ELISA, Annexin V (AnV) binding of externalized phosphatidylserine and the mitochondrial probe JC-1 using flow cytometry. Generation of reactive oxygen species (ROS) was detected using CMH2DCFDA. Western blots analysis was performed using antibodies against the pro-caspase-3, or PRAP (Poly (ADP-ribose) polymerase).The results showed that δ-elemene exhibited a marked antiproliferative effect on Hela cells in dose and time-dependent manners, and had little inhibition to normal human liver cell line WRL-68. It was demonstrated that δ-elemene was capable of inducing DNA fragmentation in a dose- and time-dependent manner. AnV positivity and the disturbance of the polarized mitochondrial transmembrane potential (Δψ_m) suggested that δ-elemene induced apoptotic death of Hela cells. Western blot analysis demonstrated that δ-elemene activated the caspase-signaling pathway, leading to the proteolysis conversion of pro-caspase-3 to activate caspase-3, and the subsequent cleavage of the caspase substrate PARP. Further, it was noted that the apoptotic effect of δ-elemene could be attenuated by L-Glutathione (GSH) or z-DEVD-fmk. It suggested that the increase in ROS generation might be involved in the mechanism of δ-elemene induced cell apoptosis.
- 公益社団法人日本薬学会の論文
- 2006-10-01
著者
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Wang Min-wei
Department Of Pharmacology Shenyang Pharmaceutical University School Of Pharmacy
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LI Ming
Department of Chemistry, Kyungpook National University
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YANG Wei
Department of Materials Science & Engineering
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Yang Wei
Dep. Of Pharmacology Shenyang Pharmaceutical Univ.
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TAO Shu-Juan
Department of Chemical Pharmaceutics, Shenyang Pharmaceutical University
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DONG Jin-Hua
Department of Chemical Pharmaceutics, Shenyang Pharmaceutical University
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WANG Xi-Sha
Department of Pharmacology, Shenyang Pharmaceutical University
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LI Karen
Department of Paediatric, Prince of Wales Hospital, Chinese University of HongKong
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Li Karen
Department Of Paediatric Prince Of Wales Hospital Chinese University Of Hongkong
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Wang Xi-sha
Department Of Pharmacology Shenyang Pharmaceutical University:department Of Medicine Chinese Univers
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Dong Jin-hua
Department Of Chemical Pharmaceutics Shenyang Pharmaceutical University
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Tao Shu-juan
Department Of Chemical Pharmaceutics Shenyang Pharmaceutical University
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Li Ming
Department Of Bioproductive Science Faculty Of Agriculture Utsunomiya University
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Yang Wei
Department Of Hematology Division Of Thrombosis And Hemostasis Jichi Medical School
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Wang Min-wei
Department Of Pharmacology Shenyang Pharmaceutical University
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Wang Min-wei
Department Of Pharmacology Shen Yang Pharmaceutic University
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LI Ming
Department of Medicine, Chinese University of HongKong
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Li Ming
Department of Anatomy & Neurobiology, School of Basic Medicine, Central South University
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