B-Cell Lymphoma-2 Over-Expression Protects δ-Elemene-Induced Apoptosis in Human Lung Carcinoma Mucoepidermoid Cells via a Nuclear Factor Kappa B-Related Pathway

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概要

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• δ-Elemene, an antitumor component, is a chemical compound isolated from Curcuma wenyujin, a Chinese traditional herb. We examined whether δ-elemene could affect apoptosis in human lung carcinoma mucoepidermoid NCI-H292 cells, and test whether and how the over-expression of B-cell lymphoma-2 (Bcl-2) and B-cell lymphoma extra large (Bcl-xL) could off-set the effect of δ-elemene on cell growth. The result demonstrated that δ-elemene significantly induced apoptosis of NCI-H292, as shown by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, DNA fragmentation measurement, Annexin V (AnV) binding of externalized phosphatidylserine and the mitochondrial probe JC-1 using flow cytometry. Treatment of NCI-H292 with δ-elemene increased both p38 mitogen-activated protein kinase (MAPK) and inducible nitric oxide synthese (iNOS) levels, suggesting these two molecules maybe relate to the apoptotic effect of δ-elemene. The cells with Bcl-2 or Bcl-xL over-expression showed an elevation of nuclear factor kappa B (NF-kappa B) activity, accompanying a significant reduction of δ-elemene-induced apoptosis. Furthermore, inhibition of NF-kappa B by IkBαSR, which is a powerful inhibitor of NF-kappa B, restored the ability of δ-elemene to induce apoptosis in the cells transfected with Bcl-2. These data strongly indicated that the apoptotic effect of δ-elemene on NCI-H292 was closely associated with the activity of NF-kappa B, which was up-regulated by Bcl-2 and Bcl-xL. In conclusion, δ-elemene induced apoptosis in NCI-H292 cells. The apoptotic effect of δ-elemene could be significantly offset by over-expression of either Bcl-2 or Bcl-xL. Bcl-2 and Bcl-xL were able to increase the activity of NF-kappa B, which was a known anti-apoptotic molecule in human lung cancer cells.

著者

• Yang Wei

  Dep. Of Pharmacology Shenyang Pharmaceutical Univ.

• YANG Wei

  Center for Drug Non-clinical Research and Evaluation of Pharmaceutical Industrial Research Institute

• YING Jun

  Center for Drug Non-clinical Research and Evaluation of Pharmaceutical Industrial Research Institute

• LI Karen

  Prince of Wales Hospital, Chinese University of Hong Kong

• Xie Chun

  The Sixth Affiliated Hospital, Sun Yat-Sen University

• Ni Qing

  Center for Drug Non-clinical Research and Evaluation of Pharmaceutical Industrial Research Institute

• Pan Xue

  College of Pharmacy, GuangDong Pharmaceutical University

• Dong Jin

  Department of Chemical Pharmaceutics, Shenyang Pharmaceutical University

• Wang Xi

  College of Pharmacy, GuangDong Pharmaceutical University

• YING Jun

  Center for Drug Non-Clinical Research and Evaluation of Pharmaceutical Industrial Research Institute in Guangzhou

• YANG Wei

  Center for Drug Non-Clinical Research and Evaluation of Pharmaceutical Industrial Research Institute in Guangzhou

• Ni Qing

  Center for Drug Non-clinical Research and Evaluation of Pharmaceutical Industrial Research Institute in Guangzhou

関連論文

• Synthesis of N-Heteroaroyl Aminosaccharide Derivatives as Fibroblast Growth Factor 2 Signaling Modulators
• Cell Apoptosis Induced by δ-Elemene in Colorectal Adenocarcinoma Cells via a Mitochondrial-mediated Pathway
• The Effect of δ-Elemene on Hela Cell Lines by Apoptosis Induction
• LC-MS Determination and Pharmacokinetic Studies of Ursolic Acid in Rat Plasma after Administration of the Traditional Chinese Medicinal Preparation Lu-Ying Extract
• B-Cell Lymphoma-2 Over-Expression Protects δ-Elemene-Induced Apoptosis in Human Lung Carcinoma Mucoepidermoid Cells via a Nuclear Factor Kappa B-Related Pathway
• Induction of caspase-3-dependent apoptosis in human leukemia HL-60 cells by δ-elemene
• Induction of Caspase-3-Dependent Apoptosis in Human Leukemia HL-60 Cells by .DELTA.-Elemene

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