Correlation between expression of major histocompatibility complex class I and that of antigen presenting machineries in carcinoma cell lines of the pancreas, biliary tract and colon
スポンサーリンク
概要
- 論文の詳細を見る
To elicit a tumor immune response, tumor antigens represented by majorhistocompatibility (MHC) class I complex on the cell surface is indispensable. Someinvestigators demonstrated that many cancer cells reduce expression ofβ2-microglobulin, a transporter of antigen presenting (TAP) or low molecular protein(LMP), due to the deletion mutant or point mutation. We investigated gene expressionlevels of antigen presenting machineries in 13 cell lines of the pancreas, biliary tractand colon cancer by using real-time quantitative PCR. We also evaluated thecorrelation between expression of MHC class I and that of antigen-processingmolecules in these gastrointestinal cancer cell lines. Flow cytometric analysis showedthat expression of MHC class I for the pancreatic cancer cell lines was generally lowerthan that for the biliary tract or colon cancer cell lines. It was further found that thecolon cancer cell lines HCT-15/DLD-1 showed no MHC class I expression and lack ofprotein expression for β2-microglobulin. Transfection of the wild-typeβ2-microglobulin gene restored MHC class I antigen expression on the cell surface forDLD-1. Quantitative real-time PCR demonstrated lower expression for TAP1, TAP2,LMP2 and LMP7 gene in five cancer cell lines. Partial correlation analysisdemonstrated that LMP2 was the only antigen presenting machinery which wassignificantly associated with MHC class I expression. Our results suggest thatβ2-microglobulin and LMP2 are important for the expression of MHC class I in 13gastrointestinal cancer cell lines, while the combined but complex expression of antigenpresenting mechanisms was related to MHC class I expression level on the surface ofcancer cells.
- 神戸大学の論文
著者
-
Nakamura T
Department Of Gastroenterological Surgery Kobe University Graduate School Of Medical Sciences
-
Kuroda Y
Department Of Surgery Kobe University Graduate School Of Medicine
-
Kuroda Yoshikazu
神戸大学 医学研究科外科学講座
-
Kuroda Y
Division Of Gastroenterological Surgery Department Of Clinical Molecular Medicine Kobe University Gr
-
Ajiki T
Department Of Surgery Kobe University Graduate School Of Medicine
-
Ajiki Tetsuo
神戸大学 医学研究科外科学講座
-
Ajiki Tetsuo
塩野義製薬
-
TAKASE SHIRO
Department of Gastrointestinal Surgery, Kobe University Graduate School of Medical Sciences
-
Imanishi Tatsuya
Kobe University Graduate School of Medicine
-
Kamigaki Takashi
Kobe University Graduate School of Medicine
-
Nakamura Tetsu
Kobe University Graduate School of Medicine
-
Hayashi Shun
Kobe University Graduate School of Medicine
-
Yasuda Takashi
Kobe University Graduate School of Medicine
-
Kawasaki Kentaro
Kobe University Graduate School of Medicine
-
Takase Shiro
Kobe University Graduate School of Medicine
-
Ajiki Tetsuo
Kobe University Graduate School of Medicine
-
Kuroda Yoshikazu
Kobe University Graduate School of Medicine
-
Ku Y
Department Of Hepato-biliary-pancreatic Surgery Kobe University Graduate School Of Medical Sciences
-
Ku Yonson
Surgery Kobe University Graduate School Of Medicine
-
Kimura T
Kobe University Graduate School Of Medicine
-
Kamigaki T
Kobe Univ. School Of Medicine Kobe
-
Kamigaki Takashi
神戸大学 第1外科
-
Kuroda Y
神戸大学 医学研究科外科学講座
-
Kuroda Yoshikazu
Division Of Gastroenterological Surgery Department Of Clinical Molecular Medicine Kobe University Gr
-
Ajiki Tetsuo
First Department Of Surgery Faculty Of Medicine Kobe University
-
Ajiki Tetsuo
Department Of Surgery Kobe University Graduate School Of Medicine
-
Ajiki Tetsuo
Department Of Gastroenterological Surgery Kobe University Graduate School Of Medicine
-
Ajiki Tetsuo
Department Of Gastroenterological Surgery Graduate School Of Medical Sciences Kobe University
-
Nakajima Takahiro
Department Of Gastroenterological Surgery Kobe University Graduate School Of Medical Sciences
-
Takase Shiro
Department Of Gastrointestinal Surgery Kobe University Graduate School Of Medical Sciences
-
Takara Kohji
Department Of Hospital Pharmacy School Of Medicine Kobe University
-
Kuroda Yoshikazu
First Department Of Surgery Faculty Of Medicine Kobe University
-
Ku Yonson
Department Of Gastroenterological Surgery Kobe University Graduate School Of Medicine
-
Kuroda Yoshikazu
First Department Of Surgery Kobe University School Of Medicine
-
Nakamura Takeshi
Department Of Gastroenterological Surgery Kobe University Graduate School Of Medical Sciences
-
Koishi Tomokazu
Department Of Hospital Pharmacy Faculty Of Pharmaceutical Sciences Kyoto Pharmaceutical University
関連論文
- Association of Cumulative Cyclosporine Dose with Its Irreversible Nephrotoxicity in Japanese Patients with Pediatric-Onset Autoimmune Diseases(Biopharmacy)
- Prediction of systemic exposure to cyclosporine in Japanese pediatric patients
- Plasma tissue factor pathway inhibitor levels in patients with acute pancreatitis
- Utility of the new Japanese severity score and indications for special therapies in acute pancreatitis
- Long-term outcome of severe acute pancreatitis
- Prediction of early death in severe acute pancreatitis
- Role of Toll-Like Receptor 4 in the Pathophysiology of Severe Acute Pancreatitis in Mice
- Long-Term Assessments After Pancreaticoduodenectomy with Pancreatic Duct Invagination Anastomosis
- Decreased Expression of Toll-like Receptor 2 and 4 on Macrophages in Experimental Severe Acute Pancreatitis
- Treatment outcome of selective digestive decontamination and enteral nutrition in patients with severe acute pancreatitis
- Treatment strategy against infection : clinical outcome of continuous regional arterial infusion, enteral nutrition, and surgery in severe acute pancreatitis
- Immunosuppression in patients with severe acute pancreatitis
- Elevation of plasma tissue factor levels in patients with severe acute pancreatitis
- Significant elevation of serum interleukin-18 levels in patients with acute pancreatitis
- Nonocclusive Visceral Ischemia Associated with Severe Acute Pancreatitis
- Intraperitoneal Administration of Oxygenated Perfluorochemical Inhibits Bacterial Translocation Associated with Severe Acute Pancreatitis
- Splenic Atrophy in Experimental Severe Acute Pancreatitis
- Hydronephrosis Associated with Acute Pancreatitis
- Duodenal Necrosis Associated with Acute Pancreatitis
- Hepatocyte Growth Factor Increases in Injured Organs and Functions as an Organotrophic Factor in Rats with Experimental Acute Pancreatitis
- Inhibitory Effect of Vacuolating Toxin of Helicobacter pylori on Enzyme Secretion from Rat Pancreatic Acini
- Impact of internal biliary drainage after pancreaticoduodenectomy
- IV-2-3 Hand-assisted laparoscopic distal pancreatectomy : its safety and efficacy in laparoscopic pancreatic surgery(Pancreatic surgery,International Video Teleconference)
- Resection of gallbladder cancer with hepatic metastasis after chemotherapy with gemcitabine
- Decreased expression of intestinal chemokine TECK/CCL25 in experimental obstructive jaundice and its reversal following internal biliary drainage
- Antitumor Effect of Gemcitabine on Orthotopically Inoculated Human Gallbladder Cancer Cells in Nude Mice
- Intrahepatic Segmental Primary Sclerosing Cholangitis : Report of a Case
- Correlation between expression of major histocompatibility complex class I and that of antigen presenting machineries in carcinoma cell lines of the pancreas, biliary tract and colon
- Carcinosarcoma of the gallbladder with chondroid differentiation
- Reversal Effects of Ca2+ Antagonists on Multidrug Resistance via Down-regulation of MDR1 mRNA
- Three-Dimensional, but not Two-Dimensional, Culture Results in Tumor Growth Enhancement after Exposure to Anticancer Drugs
- Knock-down of Sorcin Induces Up-regulation of MDR1 in HeLa Cells(Molecular and Cell Biology)
- Effects of Reactive Oxygen Species on Cell Proliferation and Death in HeLa Cells and Its MDR1-Overexpressing Derivative Cell Line
- Effects of carvedilol on MDR1-mediated multidrug resistance : comparison with verapamil
- Effects of St John's Wort and Hypericin on Cytotoxicity of Anticancer Drugs
- Cytotoxic Effects of 27 Anticancer Drugs in HeLa and MDR1-Overexpressing Derivative Cell Lines
- Antiproliferative Activity of Rhinacanthus nasutus (L.) KURZ Extracts and the Active Moiety, Rhinacanthin C(Pharmacology)
- High Expression of Thymidylate Synthase Leads to Resistance to 5-Fluorouracil in Biliary Tract Carcinoma in vitro
- MDR1 T-129C Polymorphism can be Predictive of Differentiation, and Thereby Prognosis of Colorectal Adenocarcinomas in Japanese(Biopharmacy)
- MDR1, MRP1 and MRP2 Genotypes and In Vitro Chemosensitivity in Japanese Patients with Colorectal Adenocarcinomas
- Intradermal Concentration of Hydroquinone after Application of Hydroquinone Ointments Is Higher than Its Cytotoxic Concentration(Biopharmacy)
- Different Contribution of CYP2C19 in the in Vitro Metabolism of Three Proton Pump Inhibitors
- A Multi-institutional Study on Adjuvant Chemotherapy for the Prevention of Post-operative Liver Metastases in Colorectal Cancer Patients : Comparison Between Hepatic Arterial and Portal Adriamycin Plus Oral UFT Administration
- Combined Gastrectomy with Adjacent Organs in T4 Gastric Cancer : Therapeutic Results and Indication
- Appendiceal Intussusception Induced by Tubulovillous Adenoma with Carcinoma in Situ : Report of a Case
- Immunohistochemical P 53 Protein Expression in Advanced Colorectal Cancer Lesions : No Association with Prognostic Variables or Survival
- Isolated Gastric Varices Resulting from Iatrogenic Splenic Vein Occlusion : Report of a Case
- Isolated hepatic perfusion chemotherapy for unresectable malignant hepatic tumors
- Possible Role of Heat Shock Protein 60 in Reducing Ischemic-Reperfusion Injury in Canine Pancreas Grafts After Preservation by the Two-Layer Method
- Superiority of Mild Hypothermic (20℃) Preservation for Pancreatic Microvasculature Using the Two-Layer Storage Method
- Fibromatoses of multicentric origin : a case report
- Evidence of Protein Synthesis During Resuscitation of Ischemically Damaged Canine Pancreas by the Two-Layer Method
- Recovery of Pancreatic Tissue Perfusion and ATP Tissue Level After Reperfusion in Canine Pancreas Grafts Preserved by the Two-Layer Method
- Centrifugal Pump-Assisted Venous Bypass Between the Superior Mesenteric Vein and the Umbilical Vein During Portal Vein Resection
- Resected Acinar Cell Carcinoma of the Pancreas with Tumor Thrombus Extending into the Main Portal Vein : Report of a Case
- Percutaneous Hepatic Venous Isolation and Extracorporeal Charcoal Hemoperfusion for High-Dose Intraarterial Chemotherapy in Patients with Colorectal Hepatic Metastases
- A Novel Paper Cuff for Vascular Reconstruction in Canine Liver Transplantation
- Adenine Nucleotide Metabolism of Canine Pancreas Graft Preserved by the Two-Layer (Euro-Collins' Solution/Perfluorochemical) Method after Reperfusion
- Suppression of Human Pancreatic Carcinoma Cell Growth and Invasion by Epigallocatechin-3-Gallate
- A Novel System for Small Bowel Preservation - Cavitary Two-Layer (University of Wisconsin Solution/Perfluorochemical) Cold Storage Method -
- Intraoperative Continuous External Bile Drainage During Pancreaticoduodenectomy
- MDR1 Haplotype Frequencies in Japanese and Caucasian, and in Japanese Patients with Colorectal Cancer and Esophageal Cancer
- MDR1 C3435T Polymorphism Is Predictive of Later Onset of Ulcerative Colitis in Japanese(Biopharmacy)
- Reversal Effects of Antifungal Drugs on Multidrug Resistance in MDR1-Overexpressing HeLa Cells
- Negative immunohistochemical staining of p53 protein does not always reflect wild-type p53 gene in cancer cells
- Short-term effects of external and internal biliary drainage on liver and cellular immunity in experimental obstructive jaundice
- IL-1β Genotype-Related Effect of Prednisolone on IL-1β Production in Human Peripheral Blood Mononuclear Cells under Acute Inflammation(Pharmacology)
- Genetic polymorphisms associated with adverse events and elimination of methotrexate in childhood acute lymphoblastic leukemia and malignant lymphoma
- Effect of Therapeutic Moderate Hypothermia on Multi-drug Resistance Protein 1-Mediated Transepithelial Transport of Drugs
- 急性炎症下におけるヒト末梢血単核球MDR1の遺伝子型に依存した遺伝子発現量ならびに機能低下
- Comparison of Synthetic DNA Templates with Authentic cDNA Templates in Terms of Quantification by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction(Analytical Biochemistry)
- Effects of Acid and Lactone Forms of Eight HMG-CoA Reductase Inhibitors on CYP-Mediated Metabolism and MDR1-Mediated Transport
- 電流検出方式DNAチップを用いた複数遺伝子型同時判定法のMDR1遺伝子への応用
- Usefulness of Performing a Pancreaticojejunostomy with an Internal Stent After a Pancreatoduodenectomy
- Palliative pancreatectomy with postoperative gemcitabine for patients with advanced pancreatic cancer
- Effects of Polymorphisms of MDR1, MRP1, and MRP2 Genes on Their mRNA Expression Levels in Duodenal Enterocytes of Healthy Japanese Subjects(Biopharmacy)
- MDR1-Mediated Interaction of Digoxin with Antiarrhythmic or Antianginal Drugs
- Factors Influencing the Prediction of Steady State Concentrations of Digoxin
- Interaction of Docetaxel ("Taxotere") with Human P-Glycoprotein
- P-gp誘導 Caco-2 細胞 : P-gp高感度 Assay System の構築
- N-Acetyltransferase2 Genotype Correlated with Isoniazid Acetylation in Japanese Tuberculous Patients
- Effects of Various Storage Conditions and Alterations of Antioxidant Contents on Chromatic Aberration of Hydroquinone Ointment(Biopharmacy)
- Effect of Serum Triglyceride Concentration on the Fluctuation of Whole Blood Concentration of Cyclosporin A in Patients
- Intraperitoneal Injection of Oxygenated Perfluorochemical Improves the Outcome of Intraportal Islet Transplantation in a Rat Model
- Proapoptotic Effect of a Dietary Supplement : Water Soluble Chitosan Activates Caspase-8 and Modulating Death Receptor Expression
- N-Acetyltransferase 2 Genotype-Related Sulfapyridine Acetylation and Its Adverse Events
- Optimal Dose of Omeprazole for CYP2C19 Extensive Metabolizers in Anti-Helicobacter pylori Therapy : Pharmacokinetic Considerations
- N-Acetyltransferase 2 Genotype Correlates with Sulfasarazine Pharmacokinetics after Multiple Dosing in Healthy Japanese Subjects
- Pharmaceutical and Clinical Assessment of Hydroquinone Ointment Prepared by Extemporaneous Nonsterile Compounding
- Molecular and Pharmacokinetic Properties of 222 Commercially Available Oral Drugs in Humans
- The Novel Anticancer Drug KRN5500 Interacts with, but is Hardly Transported by, Human P-Glycoprotein
- Mucinous Cystic Neoplasm of the Pancreas Presenting with Hemosuccus Pancreaticus : Report of a Case
- Novel assessment of hepatic iron distribution by synchrotron radiation X-ray fluorescence microscopy
- Pancreas Transplantation Using Non-Suture Cuff Technique in the Neck
- Complete agenesis of the dorsal pancreas
- バルプロ酸誘発高アンモニア血症に対するCPS1 4217C>A遺伝子多型の影響
- MDR1 Genotype-Related Pharmacokinetics and Pharmacodynamics
- The effectiveness of particle radiotherapy for hepatocellular carcinoma associated with inferior vena cava tumor thrombus
- Retinoic acid-induced neuronal differentiation regulates expression of genes encoding neurotrophins and their receptors in a human embryonal carcinoma cell line NTera2
- Repeating regional acute pancreatitis in the head of the pancreas caused by intraductal papillary mucinous neoplasms in the tail : report of a case