Assay of Chick Interferon Activity in Chick Kidney Cell Culture

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概要

• 論文の詳細を見る

• The availability of chick kidney cells (CK) for the assay of chick interferon was tested. When a single preparation of interferon was titrated in cultures of both CK and chick embryo fibroblasts (CEF) by the dye uptake method with vesicular stomatitis virus as challenge, the titer in 50% dye uptake (DU_<50>) units obtained in CK was approximately 4 times (0.6 log units) higher than that obtained in CEF. The reproducibility of the assay, as well as its sensitivity, was satisfactory, with the variation coefficient of approximately 10%. From these experimental data, it is concluded that the cultures of CK can well be used for the assay of chick interferon in place of those of CEF.

• 大阪府立大学の論文
• 1981-03-31

著者

• Bito Yukio

  Laboratory Of Aminal Microbiology College Of Agriculture

• HIRONAO Takeshi

  Laboratory of Animal Microbiology, College of Agriculture

• IWAMURA Shokichi

  Animal Quarantine Service, Ministry of Agriculture, Forestry and Fishery

• Hironao Takeshi

  Laboratory Of Animal Microbiology College Of Agriculture

• Iwamura Shokichi

  Animal Quarantine Service Ministry Of Agriculture Forestry And Fishery

関連論文

• Differential Susceptibilities to the Cytotoxic Effect of Anti-Bursa Cell Serum of Isotype-Specific Adoptive Immune Responses by B-Cells Early Peripheralized
• Low Density in Surface Immunoglobulin of B-Cells Early Peripheralized and Decreased in Amount of Surface Antigen(s) Specific for B-Cells
• Systemic IgA Antibody Responses against Bovine Serum Albumin in the Chicken
• Diverse Influences of Treatment of Neonatal Chickens with Testosterone-propionate on Humoral Immune Responses
• The B Cell Development Independent of the Bursa of Fabricius but Dependent upon the Thymus in Chickens Treated with Testosterone-propionate
• The Delineation of Bursal Large Round Cells Increased by Neonatal Cyclophosphamide-Treatment of Chickens and the Role of the Cells for the B Cell Maturation
• A Simple Method for Detecting Cells Producing Antibodies of Specific Immunoglobulin Classes
• The Heterogeneity of Chicken IgG
• Assay of Chick Interferon Activity in Chick Kidney Cell Culture
• Differences in Properties of Antibodies Produced by Primary Immunization and by Hyperimmunization
• Rapid Occurrence of Splenomegaly Subsequent to the Immunization of Chickens with Sheep Red Blood Cells
• T Cell-Dependence of Immature B Cells Bearing Immunocompetences Restricted to IgM-Type
• The Development of Unusual B Cell Functions in the Testosterone-propionate-Treated Chicken

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