Differences in Properties of Antibodies Produced by Primary Immunization and by Hyperimmunization

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IgG antibodies against bovine serum albumin were isolated by gel filtration and affinity chromatography from antisera harvested from chickens 9 days after primary immunization and from hyperimmunized chickens. Antibodies isolated from hyperimmunized chickens (hyper antibody) were only slightly larger in amount than those produced in an initial stage (primary antibody) in spite of much higher agglutination titres. The qualities of the antibodies were evaluated in terms of the specific activity defined as the serological activity of a fixed amount of the antibody. Hyper antibody exhibited a markedly high specific activity in comparison with primary antibody. Stepwise pH-gradient elution of antibodies from an antibody-combined immunoadsorbent column led to fully meaningful fractionation. A considerable portion of hyper antibody population required strong acid or alkali for elution. In contrast, the majority of primary antibody was eluted with weak acid or alkali. The ratio of antibody/antigen in a precipitate was significantly higher in hyper antibody than in primary antibody. Primary antibody did not precipitate chymotrypsin-digest of bovine serum albumin, which was precipitated with hyper antibody in physiological saline. Precipitation with primary antibody was efficiently suppressed by adding chymotrypsin-digest of bovine serum albumin, to which the precipitating activity of hyper antibody was virtually insensitive. Sixty-one and ninety-three per cent of primary and hyper antibodies, respectively, combined with the digest components in an immunoadsorbent column.
大阪府立大学の論文
1978-03-31

Differences in Properties of Antibodies Produced by Primary Immunization and by Hyperimmunization

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