Mitogen 刺激単核球より産生される平滑筋収縮増強因子

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Incubation of human mononuclear cells with the mitogen (PHA) led to elaboration into the supernatant of a substance which enhances the contractile responsiveness of longtuidinal smooth muscle of guinea-pig ileum to histamine and of smooth muscle of guinea-pig, cat trachea to acetylcholine. The contraction augmenting factor (CAF) was dialysable and heat stable, and the activity was not affected by atropine, tetrodotoxine or FPL 55712. The production of the factor was inhibited by indomethacin. The analysis by Thin-Layer Chromatography (TLC) showed that CAF was developed at the spot of Rf = 0.11 and the spot was lower than those of classical prostaglandins. CAF is reacted with ninhydrin, a peptide or amino-detecting reagent. The RIA analysis showed that small amount of PGs was contained in the extract from Rf=0.11. These result indicate that CAF is an peptide-containing arachidonic acid derivative of the cyclooxigenase pathway.
神戸大学の論文