Effects of Fura-2 and Indo-1 on [Ca^<++>]i in Spontaneously Beating Cultured Heart Cells

スポンサーリンク

概要

• 論文の詳細を見る

• Changes in contractility and intracellular free Ca ([Ca^<++>]i) were measured in spontaneously beating cultured heart cells following exposure to indo-1 acetoxymethyl ester (indo-1AM) and fura-2 acetoxymethyl ester (fura-2AM). Although a 10μM concentration of indo-1AM slightly decreased the beating rate, 5 and 10μM concentrations of fura-2AM produced arrest of contraction within 10 min. However, contractility returned to normal after washing. With indo-1, the diastolic and peak systolic levels of [Ca^<++>]i were 282±23 and 1107±122 nM, respectively, and 84±8 and 582±93 nM, respectively, with fura-2. The difference between the indo-1 and fura-2 generated responses are statistically significant (p<0.01). These results demonstrate that indo-1 and fura-2 can affect [Ca^<++>]i, but the influence of indo-1 is less than that of fura-2 in the heart cells.

• 東海大学の論文

著者

• Nakamura Haruo

  1st Department of Internal Medicine, National Defense Medical College

• Ishida Hideyuki

  Department of Medicine, National Defense Medical College

• Okino Haruka

  Department Of Physiology Tokai University

• Okino Haruka

  Department Of Physiology 2 School Of Medicine Tokai University

• Aosaki Noboru

  National Kasumigaura Hospital

• Sakata Nobuhiro

  1st Department Of Medicine National Defense Medical College

• Nakamura Haruo

  Department Of Medicine I National Defense Medical College

• Aosaki Noboru

  1st Dpt, of Int. Med., Natl, Def, Med. Coll.

• Ohsuzu Fumitaka

  Department Of Medicine I National Defense Medical College

• Ohsuzu Fumitaka

  First Division Department Of Internal Medicine National Defence Medical College

• Aosaki Noboru

  Department Of Medicine National Defense Medical College

• SEGUCHI Hidetaka

  1st Department of Medicine, National Defense Medical College

• OHUSUZU Fumitaka

  1st Department of Medicine, National Defense Medical College

• Shige Hideki

  First Department Of Internal Medicine National Defense Medical College

• Ishida Hideyuki

  Department Of Physiology 2 School Of Medicine Tokai University

• Nakamura Haruo

  1st Department Of Medicine National Defense Medical College

• Ohsuzu Fumitaka

  1st Department Of Medicine National Defense Medical College

• Ohsuzu Fumitaka

  Internal Medicine National Defense Medical College

• Nakamura Haruo

  1st Department Of Internal Medicine National Defense Medical College

• Ishida Hideyuki

  Department Of Digestive Tract And General Surgery Saitama Medical Center Saitama Medical University

• Seguchi Hidetaka

  1st Department Of Medicine National Defense Medical College

• Ishida Hideyuki

  Department of Chemistry, Faculty of Engineering Science, Osaka University

関連論文

• Fetal and Neonatal Development of Ca2+ Transients and Functional Sarcoplasmic Reticulum in Beating Mouse Hearts
• Incarcerated Recurrent Rectal Prolapse with Ileal Strangulation : Report of a Case
• -P79-EVALUATON OF MYOCARDIAL DAMAGE AFTER 60 MINUTES CORONARY OCCLUSION USING MYOCARDIAL CONTRAST ECHOCARDIOGRAPHY : Echocardiography, Pulsed Doppler : FREE COMMUNICATIONS(III) : PROCEEDINGS OF THE 53th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATIO
• CHANGES OF PLATELET FUNCTION AND PROSTAGLANDIN METABOLISM DURING TREADMILL TESTING AND IT'S CORRELATIONS WITH TREADMILL EXERCISE SCORE MEASURED BY DSA METHOD : Exercise Test (II) : FREE COMMUNICATIONS (VI) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC MEETI
• -P79-EVALUATON OF MYOCARDIAL DAMAGE AFTER 60 MINUTES CORONARY OCCLUSION USING MYOCARDIAL CONTRAST ECHOCARDIOGRAPHY : Echocardiography, Pulsed Doppler : FREE COMMUNICATIONS(III) : PROCEEDINGS OF THE 53th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATIO
• CHANGES OF PLATELET FUNCTION AND PROSTAGLANDIN METABOLISM DURING TREADMILL TESTING AND IT'S CORRELATIONS WITH TREADMILL EXERCISE SCORE MEASURED BY DSA METHOD : Exercise Test (II) : FREE COMMUNICATIONS (VI) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC MEETI
• EFFECTS OF HYPOXIA ON BEATING RATE AND CELL INJURIES IN CULTURED MYOCARDIAL CELL : WITH SPECIAL REFERENCE TO ATP CONTENT : Cardiac Metabolism (I) : 48 Annual Scientific Meeting, Japanese Circulation Society
• BETA-ADRENERGIC RECEPTORS IN THE MYOCARDIUM OF SPONTANEOUSLY HYPERTENSIVE RATS : Hypertension (III) : III : 48 Annual Scientific Meeting, Japanese Circulation Society
• EVALUATION OF CONTRACTION VELOCITY IN SINGLE CULTURED MYOCARDIAL CELLS : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Myocardial Metabolism Biochemistry
• EFFECTS OF PROSTANOIDS ON CULTURED MYOCARDIAL CELLS : Myocardial metabolism . Biochemistry : 46th Annual Scientific Meeting, Japanese Circulation Society
• PE-011 The maintenance of intracellular Ca2+ overload in the auto-immunized hypertrophied cardiomyopathy rabbit model(Arrhythmia, Basic 3 (A) : PE2)(Poster Session (English))
• Effect of Chronic Autoimmunity against the Second Extracellular Loop of Beta1-Adrenergic Receptors to Inward Currents in Ventricular Myocytes
• PJ-306 Effects of Lipid-Lowering Therapy with Atorvastatin on Thoracic and Abdominal Aortic Plaques : Two Years' Follow-up by MRI(Atherosclerosis, clinical-09, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• A Case of Acute Massive Pulmonary Thromboembolism Treated by Mechanical Clot Fragmentation Using a Percutaneous Transluminal Angioplasty Balloon
• Primitive Neuroectodermal Tumor Arising in the Colon : Report of a Case
• Plasma Levels of Big Endothelin-1 in Patients with Colorectal Cancer : Correlation with Liver Metastasis
• Impact of Oral Antimicrobial Prophylaxis on Surgical Site Infection and Methicillin-Resistant Staphylococcus aureus Infection After Elective Colorectal Surgery, Results of a Prospective Randomized Trial
• OJ-229 Pre-procedural Plasma Osteopontin (OPN) Levels and Cardiovascular Events in Patients Undergoing Percutaneous Coronary Intervention (PCI)(Restenosis, basic/clinical-2 (IHD) OJ39,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Mee
• HETEROGENEOUS RESPONSE OF REGIONAL WALL MOTION OF NORMAL LEFT VENTRICLE WITH RIGHT ATRIAL PACING USING TWO DIMENSIONAL ECHOCARDIOGRAPHY : Echocardiography (I) : FREE COMMUNICATIONS (III) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE
• DIFFERENT RETURN OF CARDIAC FUNCTION WITH GRADUAL AND SUDDEN REPERFUSION AFTER BRIEF CORONARY OCCLUSION : Electrophysiology : 46th Annual Scientific Meeting, Japanese Circulation Society
• Carcinosarcoma of the Rectosigmoid Colon : Report of a Case
• Pseudo-Meigs' Syndrome Caused by Ovarian Metastasis from Colon Cancer : Report of a Case
• 0591 DEVELOPMENTAL CHANGES OF SYMPATHETIC INNERVATION AND β-ADRENERGIC RECEPTOR DENSITY IN THE RAT MYOCARDIUM
• Sweet Elements of Siraitia Grosvenori Inhibit Oxidative Modification of Low-Density Lipoprotein
• 11) INCIDENCES OF CORONARY ARTERY STENOSIS AND MEAN QRS VECTOR ON THE FRONTAL PLANE VECTORCARDIOGRAM IN PATIENTS WITH INFERIOR MYOCARDIAL INFARCTION
• OJ-080 Associations between Plasma C-reactive Protein (CRP) and Osteopontin (OPN) Levels and the Severities of Aortic and Coronary Atherosclerosis(OJ14,Atherosclerosis (Clinical/Diagnosis) (IHD),Oral Presentation (Japanese),The 73rd Annual Scientific Meet
• Prognostic Significance of Plasma Osteopontin Levels in Patients Undergoing Percutaneous Coronary Intervention(Ischemic Heart Disease)
• FRS-104 Associations between Plasma Osteopontin (OPN) Levels and the Severity of Aortic and Coronary Atherosclerosis(Clinical Frontiers in Atherosclerosis(IHD),Featured Research Session,The 72nd Annual Scientific Meeting of the Japanese Circulation Societ
• Telmisartan Enhances Cholesterol Efflux from THP-1 Macrophages by Activating PPARγ
• Plasma Matrix Metalloproteinase-8 Concentrations are Associated With the Presence and Severity of Coronary Artery Disease
• Polymorphism of the 3'-Untranslated Region of Interleukin-12 p40 Gene is not Associated With the Presence or Severity of Coronary Artery Disease
• High Plasma Levels of Osteopontin (OPN) in Patients With Restenosis after Percutaneous Coronary Intervention (PCI)(Restenosis after Angioplasty, Basic/Clinical 5 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• OJ-478 Plasma Matrix Metalloprotease (MMP)-8 Levels Are Associated With the Presence and Severity of Coronary Artery Disease (CAD)(Angina Pectoris, Clinical 3 (IHD) : OJ58)(Oral Presentation (Japanese))
• Effects of Lipid-Lowering by Atorvastatin on Atherosclerotic Plaques in Thoracic and Abdominal Aorta Detected by MRI
• Protective Effect of Green Tea Consumption Against Myocardial Infarction (MI)
• Imaging Techniques for Measuring Adipose-tissue Distribution in the Abdomen: A Comparison between Computed Tomography and 1.5-Tesla Magnetric Resonance Spin-echo Imaging
• Relation of Abdominal and Thigh Adipose Tissue Distribution to Serum Lipids and Glucose Metabolism in Obese Males
• Effect of Alcohol Intake on the Levels of Plasma Homocysteine in Healthy Males
• A MECHANICAL MODEL OF AORTIC VALVULAR INSUFFICIENCY USING CANINE PAPILLARY MUSCLE : Cardiac Function (III) : III : 48 Annual Scientific Meeting, Japanese Circulation Society
• IODINE 125-PHENYLPENTADECANOIC ACID AND ITS BETA-METHYL SUBSTITUTE METABOLISM IN CULTURED MOUSE EMBRYONAL MYOCYTES : Iodine-labelled Fatty Acids as Tracers of Myocardial High Energy Phosphate
• -145-SIMULTANEOUS MEASUREMENT OF ACTION POTENTIAL AND CONTRACTION VELOCITY IN CULTURED CARDIAC CELLS
• -0865-THE EFFICACY OF ARGON LASER-MEDIATED HOT BALLOON ANGIOPLASTY : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• -0755-THE UTILITY OF PERCUTANEOUS TRANSLUMENAL CORONARY ANGIOSCOPE WITH OVER THE GUIDE WIRE SYSTEM (OGS)
• -0703-RISK OF THROMBOEMBOLISM BY GUIDWIRES FOR PTCA
• -0186-COMPARISON OF FINDINGS BETWEEN CORONARY ANGIOGRAPHY AND ANGIOSCOPY
• Important Regulatory Role of Activated Platelet-Derived Procoagulant Activity in the Propagation of Thrombi Formed Under Arterial Blood Flow Conditions(Vascular Medicine)
• OJ-047 Role of ADP Receptors P2Y_1 and P2Y_ in the Maintenance of Stability of Platelet Thrombi under Blood Flow Conditions.(Antithrombofic Ttherapy (H) : OJ6)(Oral Presentation (Japanese))
• Imaging of Structural Changes in Endothelial Cells and Thrombus Formation at the Site of FeCl_3-Induced Injuries in Mice Cremasteric Arteries
• -457-PERCUTANEOUS TRANSLUMINAL CORONARY ANGIOSCOPY : USEFULNESS AND COMPLICATIONS : Ischemic Heart Disease I : FREE COMMUNICATIONS(II) : PROCEEDINGS OF THE 53th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• -P78-STUDY OF MYOCARDIAL CONTRAST WASHOUT "T1/2" USING LOW OSMORALITY CONTRAST AGENT IOPAMIDOL : Echocardiography, Pulsed Doppler : FREE COMMUNICATIONS(III) : PROCEEDINGS OF THE 53th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• Multiple Inflammatory Pseudotumors Mimicking Liver Metastasis from Colon Cancer : Report of a Case
• A Case of Breast Cancer Diagnosed by Inguinal Lymph Node Metastasis
• ENHANCED MYOCARDIAL ADENYLATE CYCLASE ACTIVITY IN SPONTANEOUSLY HYPERTENSIVE RATS
• Effects of Fura-2 and Indo-1 on [Ca^]i in Spontaneously Beating Cultured Heart Cells
• -1271-AN EFFECT OF EXTERNAL K ON Ca-H INTERACTION IN CULTURED HEART CELLS : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• -0890-EFFECTS OF HUMAN SUPEROXIDE DISMUTASE ON ISCHEMIC AND REPERFUSED MYOCARDIUM IN ISOLATED PERFUSED RAT HEART : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• -0568-SEVERE MYOCYTE ATP DEPLETION OR Ca OVERLOAD PREDUCE IRREREVERSIBLE CELL INJURY IN CULTURED HEART CELLS
• -0360-EFFECTS OF PDGF AND INSULIN ON LOW DENSITY LIPOPROTEIN METABOLISM AND PROLIFERATION IN CULTURED MEDIAL SMOOTH MUSCLE CELLS
• INTERRELATIONSHIP BETWEEN IODINE 125PENTADECANOIC ACID AND ITS BETA-METHYL SUBSTITUTE METABOLISM AND MYOCARDIAL HIGH ENERGY PHOSPHATE : RI : 53 Annual Scientific Meeting, Japanese Circulation Society
• EFFECTS OF DILTIAZEM ON ACETYLATED LOW DENSITY LIPOPROTEIN METABOLISM IN CULTURED MOUSE PERITONEAL MACROPHAGES : Arteriosclerosis : 53 Annual Scientific Meeting, Japanese Circulation Society
• -P169- EFFECTS OF CALCIUM ANTAGONIST AND FREE RADICAL SCAVENGERS ON ISCHEMIC AND REPERFUSED MYOCARDIUM
• A COMPARISON OF LEFT VENTRICULAR HEMODYNAMIC RESPONSE IN PATIENTS OF NORMAL CORONARY GROUP WITH ANGINA PECTORIS AND CORONARY ARTERY DISEASE GROUP DURING ATRIAL PACING : Ischemic Heart Disease : PROCEEDINGS OF THE 44th ANNUAL SCIENTIFIC MEETING OF THE JAPA
• The Link Between Free Radicals and Myocardial Injury : SYMPOSIUM ON PATHOPHYSIOLOGY IN THE EARLY STAGE OF ACUTE MYOCARDIAL INFARCTION AND ITS TREATMENT : 51th Annual Scientific Session of the Japanese Circulation Society
• Effects of Soy Protein Isolate(SPI)and Casein on the Postprandial Lipemia in Normolipidemic Men
• -0162- OXIDATIVE SUSCEPTIBILITY OF LOW DENSITY LIPOPROTEIN IN PATIENTS WITH NON INSULIN DEPENDENT DIABETES MELLITUS(PROCEEDINGS OF THE 59th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY)
• Changes in the Concentration and Distribution of Lipoprotein(a) in Plasma after Fat Intake
• REMNANT LIKE PARTICLES(RLP),WHICH WAS ISOLATED BY USING IMMUNOAFFINITY GEL MIXTURE TO APO B100 AND APO A-1 FROM HYPERTRIGLYCERIDEMIC PATIENTS, SUPPRESSED HMG COA REDUCTASE ACTIVITY OF HUMAN SKIN FIBROBLASTS
• Anorectal Agenesis with a Rectourethral Fistula Diagnosed in an Adult : Report of a Case
• The Quantitative Appraisal of Coronary Vascular Lesions in Experimental Hypertension and Hypercholesterolemia with Special Reference to Intramural Arteriolosclerosis
• OE-025 Cardioprotection by Erythropoietin is Associated with Translocation of Akt to Mitochondria and Its Interaction with the Adenine Nucleotide Translocase(Myocardial ischemia-reperfusion, basic/clinical-1 (IHD) OE5,Oral Presentation (English),The 70th
• Effects of Calcium Antagonists and Free Radical Scavengers on Myocardial Ischemic and Reperfusion Injury: Evaluation by ^P-NMR Spectroscopy : SYMPOSIUM ON PROTECTION OF ISCHEMIC MYOCARDIUM : Basic and Clinical Research
• Haloperidol Prolongs Diastolic Phase of Ca^ Transient in Cardiac Myocytes
• Difference in Propagation of Ca^ Release in Atrial and Ventricular Myocytes
• Physiological Concentration of Nitric Oxide Induces Positive Inotropic Effects through cGMP Pathway in Isolated Rat Ventricular Myocytes
• -0779-QUANTIFICATION OF NONRADIOACTIVE MICROSHPERE BY FLUORESCENCE EXCIATION ANALYSIS TO MEASURE REGIONAL BLOOD FLOW
• Limitations in Calculating Left Ventricular Volume by Two Dimensional Geometry : An Exised Canine Heart Study
• Bromodeoxyuridine Uptake by Early Liver Metastases in Rats : A Comparison of the Hepatic Artery and Portal Vein Infusion Routes
• Splenic and Pulmonary Metastases from Renal Cell Carcinoma : Report of a Case
• A Foreign Body Granuloma After Gastric Perforation Mimicking Peritoneal Dissemination of Gastric Cancer : Report of a Case
• Amebic liver abscess rupturing into the lesser omentum space
• MARKED DIFFERENCE IN ROLES OF SUPEROXIDE, NO AND PEROXYNITRITE BETWEEN MYOCYTE AND ENDOTHELIAL CELL INJURY
• Effect of calcium and magnesium on neurotoxicity and blood platinum concentrations in patients receiving mFOLFOX6 therapy : a prospective randomized study
• Stanniocalcin 1 Prevents Cytosolic Ca^ Overload and Cell Hypercontracture in Cardiomyocytes
• Stanniocalcin 1 Attenuates the Cytosolic Ca Overload And the Cell Hypercontracture Induced by Ouabain (Heart Failure, Basic 6 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• Nicorandil Attenuates FeCl_3-Induced Thrombus Formation Through the Inhibition of Reactive Oxygen Species Production(Vascular Medicine)
• Study on a New Index of Fetal Respiratory Function : a Novel Optical Analysis of the Amniotic Fluid by the Vacuum-dried Infrared Attenuated Total Reflection (ATR) Method
• Short-Term Intravenous Antimicrobial Prophylaxis in Combination with Preoperative Oral Antibiotics on Surgical Site Infection and Methicillin-Resistant Staphylococcus aureus Infection in Elective Colon Cancer Surgery : Results of a Prospective Randomized
• A Lumped Analog for the Dynamic Analysis of the Pressure-flow Relationships in the Human Brachial Artery
• Gastrointestinal Stromal Tumor Arising in the Pelvic Cavity : A Case Report
• Muscle Strength and Walking Ability After Laparoscopic Hernioplasty Versus Conventional Repair
• THE EFFECTS OF CoQ_, DEX AND MP DURING HYPOXIA : A STUDY ON THE CULTURED SINGLE MYOCARDIAL CELL : Cardiovascular drugs (I) : FREE COMMUNICATIONS (Abstract) : 45 Annual Scientific Meeting, Japanese Circulation Society
• APC gene mutations in a jejunal adenoma causing intussusception in a patient with familial adenomatous polyposis
• Serum Levels of Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) in Colorectal Cancer Patients
• Fulminant amoebic colitis with perforation successfully treated by staged surgery : a case report
• Pseudo-Meigs'syndrome caused by ovarian metastasis from rectosigmoid cancer
• Risk of Missing Colorectal Cancer During Laparoscopic Cholecystectomy
• The Facilitation of Peritoneal Dissemination of a Tumor by Laparotomy in a Rabbit Model
• A New Simple Technique for Performing Intraoperative Endoscopic Resection of Small-Bowel Polyps in Patients with Peutz-Jeghers Syndrome
• Effects of Soy Protein Isolate (SPI) and Casein on the Postprandial Lipemia in Normolipidemic Men
• Chairmen's Introduction to the Panel Discussion : PANEL DISCUSSION ON TESTING METHODS FOR CARDIOVASCULAR DIAGNOSIS : Critiques on the Analytical Methods for Cardiac Events

もっと見る 閉じる

スポンサーリンク