Important Regulatory Role of Activated Platelet-Derived Procoagulant Activity in the Propagation of Thrombi Formed Under Arterial Blood Flow Conditions(Vascular Medicine)

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Background: The functional links between the activation of platelets and the coagulation system have not been clarified. Methods and Results: Immobilized collagen fibrils were perfused with human blood containing fluoresceinated platelets in the presence of various concentrations of thrombin inhibitor. Coagulant activity around platelet thrombi was detected using a FITC-conjugated antibody against the fibrin monomer complex (F-405). Intra-cytosolic calcium ion concentrations ([Ca^<2+>]i) in individual platelets and the volume of thrombi were detected with an ultrafast confocal laser microscope equipped with a piezo-motor control unit. The volume of platelet thrombi formed after 8 min of blood perfusion in the presence of 10, 25, 50, and 100μmol/L argatroban was 7.69±0.46μm^3, 6.61±1.96μm^3, 3.63±1.54μm^3, and 1.67±0.75μm^3, respectively. There was a positive correlation between the volume of platelet thrombi and the amount of fibrin monomer complex produced around them. The [Ca^<2+>]i of the platelets forming the thrombi oscillated between a minimum of 92.0±57.4nmol/L, 120.1±68.1nmol/L, and a maximum of 217.6±131.5nmol/L, 367.6±189.1nmol/L, respectively, in the presence of 100 and 10μmol/L argatroban. Conclusions: The results suggest a crucial role of coagulant activity in both the generation of fibrin and the growth of platelet thrombi.
社団法人日本循環器学会の論文
2009-02-20