培養骨芽細胞におけるストレスファイバー構造再形成過程の観察(S02-3 生体組織のマルチスコープメカニクス(3).細部のマルチモーダル・メカニクス,S02 生体組織のマルチスコープメカニクス)

概要

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The cytoskeletal actin structure is dynamically reorganized in various cellular activities such as cell movement and cytokinesis. In addition, the change of mechanical environment also induces the reorganization of the actin structure. The aim of this study is to clarify the reorganization mechanism particularly focusing on the organizing process of actin fiber structure. Firstly, actin fiber structure is completely disassembled by Cytochalasin D treatment. After the treatment, re-assembling process of actin structure was observed. Actin fibers in living osteoblastic cells were fluorescently labeled using the transfection technique of EGFP-actin fused protein DNA. And, dynamics of cellular membrane and actin cytoskeleton was visualized using fibronectin-coated microparticles that adhered onto the cellular membrane. From the observed results, in the initial phase of the re-assembling, actin fibers were formed to connect between the microparticles and the cell nucleus. Subsequently, microparticles moved along the formed fibers toward the nucleus. Therefore, it can be predicted that not only fibronectin but also structures surrounding the cell nucleus guides the actin fiber assembly and that cell membrane is moving along to actin fibers.
社団法人日本機械学会の論文
2004-09-04