アクチン細胞骨格構造の量が培養骨芽細胞の力学刺激応答特性に及ぼす影響

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Bone responds to change in mechanical environment and adaptively remodels the internal structure and external shape. Therefore, for bone cells, such as bone-forming osteoblasts, a mechanical stimulus as well as biochemical signaling plays an important role in the regulation of their activities. Many studies on various osteoblastic responses to mechanical stimulus have been reported ; however, the mechanosensory mechanism by which cells sense the stimulus and translate it to biochemical signaling is not clearly understood. Cytoskeletal actin fibers have been proposed as one of the candidates playing an important role in the mechanosensory mechanism. In this study, we hypothesize that the organized network structure of actin fibers is essential in the mechanosensory mechanism. Three groups of osteoblas-like cells with different amounts of controlled actin fiber structure were prepared by controlling polymerization time after treatment with cytochalasin D. By applying mechanical stimulus to a single osteoblast-like cell, as localized deformation of the cytomembrane by direct indentation with a glass microneedle, a calcium signaling response to the stimulus was observed using a fluorescent calcium indicator, Fluo 4, under a confocal laser-scanning microscope. As a result, cells responded more sensitively to the applied deformation with a larger amount of actin fiber structure, and a few cells without an organized structure responded to stimulus as well. These results suggest that the organized actin fiber structure is not necessarily essential in the mechanism by which the mechanical stimulus is transduced into a calcium signaling response, but plays a supporting role indirectly, such as improving the efficiency of transmitting the stimulus to the cell or amplifying the stimulus itself.
社団法人日本生体医工学会の論文
2003-12-10

アクチン細胞骨格構造の量が培養骨芽細胞の力学刺激応答特性に及ぼす影響

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