力学的刺激に対する培養骨芽細胞内Ca^<2+>濃度変化に及ぼすアクチンフィラメントの影響

概要

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Under the influence of mechanical environment, bone structure is formed and maintained by adaptive remodeling which involves osteoclastic resorption and osteoblastic formation. In the mechanotransduction system in osteoblasts, it is believed that intracellular calcium plays fundamental roles and actin filament is a crucial component for the signal transduction processes. To clarify the role of actin filament in deformation-induced Ca^<2+> signaling, osteoblast-like cells (MC3T3-E1) with different volume fraction of actin filament controlled by cytochalasin D were subjected to tensile strain in vitro. And, the change in intracellular Ca^<2+> density labeled by fluo-3 was observed by using a laser-scanning confocal microscope. An a result, disruption of the actin filament greatly suppressed the deformation-iuduced Ca^<2+> response that was regulated according as the degree of actin filament organization. This result indicates that the actin filament is indispensable in quantitative regulation of the Ca^<2+> signaling in response to the mechanical stimulus in osteoblasts.
一般社団法人日本機械学会の論文
2001-02-25