他感作用物質検索のための発芽・生育試験の,ロジスチック関数(RICHARDS関数)を用いた解析

概要

論文の詳細を見る
A screening method for the detection of allelopathic activities by using the logistic function, RICHARDS' function, fitted to lettuce seed germination and growth curves was developed. Using this method, we were able (1)to determine the parameters of germination and growth that are biologically significant, namely, the final germination percentage (A), germinaion rate (R), and the onset of germination (Ts), (2)to determine the optimum conditions for the development of an allelopathy assay, for example, osmotic pressure, pH, temperature, light, number of seeds, (3)to estimate the mechanism of action of active substances, and (4)to quantify the activity of allelochemicals. The conditions for testing lettuce seed germination for the screening of allelopathic properties were set up as follows. A Whatman No.1 filter paper was placed in a 5.5cm (inner diameter) petri dish, and 1.6ml of the test solution was added. The osmotic pressure of the test solution should not exceed 50 kPa, which is equivalent to an electroconductivity of 1 mS/cm. Twenty seeds of lettuce (Great Lakes 366) were put on the paper, and incubated in the dark at 25℃. This method which is applicable to the seed germination and growth tests of other plants enables to evaluate chemical or physical effects.
日本雑草学会の論文
1990-12-25