69.シアナミド生合成における窒素原子および炭素原子の由来(口頭発表)

概要

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Natural cyanamide (NH_2CN) has recently proved to occur in some Leguminosae plants including hairy vetch Vicia villosa subsp. varia. In the present study, we demonstrated the incorporation of ^<15>N and ^<13>C from labeled precursors into cyanamide using shoots of V. villosa subsp. varia. We incubated the 3-week-old shoots in the presence of 5μM of H^<13>CN for 24h and in the absence for another 48h. As a result of GCMS analysis of the cyanamide isolated from the shoots, direct incorporation of the cyano group of H^<13>CN into cyanamide was not observed. By the administration of five ^<15>N-labeled amino acids, the ^<15>N isotope abundance ratio in the isolated cyanamide increased slowly and reached the maximum between 3 and 4% respectively after 4d treatments, the same as the administration of inorganic nitrogenous compounds. Administration of [2, 3-^<13>C] pyruvate enhanced the ^<13>C percentages in succinate and fumarate, while no significant incorporation of ^<13>C into cyanamide was detected. This result suggests that the carbon atom in cyanamide is derived from a hydrocarbon between glucose and pyruvate in glycolysis or on the pentose-phosphate cycle.
植物化学調節学会の論文
2008-10-06