70.植物の一生に及ぼすアレロパシー活性を検定するライフサイクルアセスメント(LCA法)の開発とDNAマイクロアレイによる影響の解析(口頭発表)

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Plants life cycle starts from germination, then early vegetative growth, maturing stage, finally regeneration stage and seed formation. We have already developed assessment methods for allelopathy during the early growth stage. The present study aims at the evaluation of Allelopathic effect in the whole life cycle of plants. Establishment of Life cycle assessment (LCA): Sterilized seeds of Arabidopsis thaliana were sown in an Agripot containing 1% agar with Murashige and Skoog Medium and 1% sucrose. Plants were maintained for 7 weeks, until seed formation. Triple plant box apparatus was made to insure the height at the maturing stage. Assessment of allelochemicals by DNA microarray in combination with LCA: Effects of allelochemicals identified in buckwheat, velvet bean and hairy vetch were tested using LCA at EC50 concentration. Fagomine, gallic acid and rutin from buckwheat and L-DOPA from velvet bean, all have catechol group, had little effect for flowering, but cyanamide from hairy vetch strongly delay flowering. After 20 days of treatment in LCA, they were transferred to water solutions containing allelochemicals for 6 hours. Total RNA was isolated using Qiagen RNeasy Plant kit, then amplified and labelled by GeneChip Expression Analysis (Affymetrix). Results were confirmed using quantitative real-time PCR. Exposure to fagomine, gallic acid, rutin and L-DOPA showed the same type of gene profile, but cyanamide showed completely different reaction.
2008-10-06

70.植物の一生に及ぼすアレロパシー活性を検定するライフサイクルアセスメント(LCA法)の開発とDNAマイクロアレイによる影響の解析(口頭発表)

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