Purification and Characterization of Dehydroascorbate Reductase from Rice

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概要

• 論文の詳細を見る

• Dehydroascorbate reductase (DHAR; EC 1.8.5.1) is an enzyme that is critical for maintenance of an appropriate level of ascorbate in plant cells. This report describes the purification and characterization of a GSH-dependent DHAR from rice (Oryza sativa) bran and is the first, to our knowledge, of such an analysis of DHAR from a monocot. The enzyme was a monomeric thiol enzyme, resembling DHARs purified from dicots, but it was different from them in terms of heat stability and antigenicity. The amino-terminal amino acid sequence of the DHAR from rice did not show any obvious similarity to those of known proteins with DHAR activity, such as, glutaredoxin (thioltrans-ferase), protein disulfide isomerase, and trypsin inhibitor. Immunoprecipitation analysis showed that this enzyme was a major DHAR in etiolated seedlings. Western blot analysis indicated that this enzyme was distributed ubiquitously in rice tissues. A similar protein was found in barley but not in dicots.

• 日本植物生理学会の論文

著者

• USHIMARU Takashi

  Department of Biology and Geoscience, Faculty of Science, Shizuoka University

• MAKI Yasushi

  Department of Physics, Osaka Medical College, Takatsuki

• KATO Yoshihiro

  Department of Biochemistry and Engineering, Faculty of Engineering, Tohoku University

• Ushimaru Takashi

  Department Of Biology Faculty Of Science Shizuoka University

• Ushimaru Takashi

  Department Of Biology And Geoscience Faculty Of Science Shizuoka University

• Maki Y

  Kyoto Univ. Kyoto Jpn

• Maki Yasushi

  Department Of Botany Faculty Of Science Kyoto University

• Kato Y

  Department Of Biology Faculty Of Science Shizuoka University

• Urano Junichi

  Department of Biology, Faculty of Science, Shizuoka University

• Urano Junichi

  Department Of Biology Faculty Of Science Shizuoka University

• Kato Yoshihiro

  Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University

• Urano Jun′ichi

  Department of Biology, Faculty of Science, Shizuoka University

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