Stoichiometric Analysis of Barley Plastid Ribosomal Proteins

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概要

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• We analyzed the protein composition of plastid 70S ribosomes isolated from the stromal fractions of barley plastids by the radical-free and highly reducing method of two dimensional polyacrylamide gel electrophoresis(RFHR 2D-PAGE). Intactness of the ribosomes was confirmed by the poly(U)-directed phenylalanine polymerization activity and by the reassociation capacity of the subunits into 70S ribosomes. The small and large ribosomal subunits were composed of 23 and 36 proteins, respectively. In addition, one acidic protein associated with ribosomes in low salt buffer but released in high salt buffer was found. The plastid ribosomes contained relatively larger numbers of acidic proteins than prodaryotic ribosomes. Stoichiometric analysis revealed the presence of several ribosomal proteins in low copy numbers, indicating that the ribosomes of plastids were heterogeneous. We also investigate the protein composition of plastid ribosomes from greening barley leaves and found that it did not change during greening.

• 日本植物生理学会の論文

著者

• Wada Akira

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya Institute Of Technology

• Maki Yasushi

  Department Of Botany Faculty Of Science Kyoto University

• Tanaka Ayumi

  Institute Of Low Temperatue Science Hokkaido University

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