The Arabidopsis-accelerated cell death gene ACD1 is involved in oxygenation of pheophorbide a: inhibition of the pheophorbide a oxygenase activity does not lead to the "stay-green" phenotype in Arabidopsis

スポンサーリンク

概要

• 論文の詳細を見る

• This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version Oxford University Press, Plant and Cell Physiology, 44(12), 2003, p1266-1274 is available online at: http://www.oxfordjournals.org/ that the author will receive upon publication here].Oxygenation of pheophorbide a is a key step in chlorophyll breakdown. Several biochemical studies have implicated that this step was catalyzed by an iron-containing and ferredoxin-dependent monooxygenase, pheophorbide a oxygenase (PaO). It has been proposed that inhibition of its activity arrests the chlorophyll breakdown and leads to the "stay-green" phenotype. We searched the Arabidopsis genome for a possible PaO-encoding gene and hypothesized that it has homology to known iron-containing Rieske-type monooxygenase sequences. We identified three such open reading frames, Tic55, ACD1 and ACD1-like. We produced transgenic Arabidopsis plants which expressed antisense RNA as a method to inhibit the expression of these genes. The appearance of these antisense plants were indistinguishable from that of the wild type under illumination. However, after they were kept under darkness for 5 d and again illuminated, the leaves of the antisense ACD1 plants (AsACD1) were bleached. Leaves of AsACD1 accumulated 387 nmol (g FW)(-1) pheophorbide a which corresponded to 60% of chlorophyll a degraded. The rate of decrease in chlorophyll a was not influenced in senesced AsACD1 leaves. These results demonstrated that ACD1 is involved in PaO activity, and its inhibition led to photooxidative destruction of the cell instead of the "stay-green" phenotype.http://www.oxfordjournals.org/

• Oxford University Pressの論文
• 2003-12-01

著者

• 田中 歩

  北海道大学低温科学研究所

• Tanaka A

  Institute For Drug Discovery Research Yamanouchi Pharmaceutical Co. Ltd.

• TANAKA Ayumi

  Institute of Low Temperature Science, Hokkaido University

• TANAKA Ryouichi

  Institute of Low Temperature Science, Hokkaido University

• Tanaka Ayumi

  Institute Of Low Temperatue Science Hokkaido University

• HIRASHIMA Masumi

  Institute of Low Temperatue Science, Hokkaido University

• SATOH Soichirou

  Institute of Low Temperature Science, Hokkaido University

• Hirashima Masumi

  Institute Of Low Temperatue Science Hokkaido University

• Tanaka Ryouichi

  Institute Of Low Temperatue Science Hokkaido University

• Satoh Soichirou

  Institute Of Low Temperature Science Hokkaido University

• 田中 歩

  Hokkaido Univ. Sapporo Jpn

関連論文

• YM-216391, a Novel Cytotoxic Cyclic Peptide from Streptomyces nobilis : II. Physico-chemical Properties and Structure Elucidation
• YM-216391, a Novel Cytotoxic Cyclic Peptide from Streptomyces nobilis : I. Fermentation, Isolation and Biological Activities
• YM-181741, a Novel Benz[a]anthraquinone Antibiotic with Anti-Helicobacter pylori Activity from Streptomyces sp.
• γ-Pyrone Compounds with Selective and Potent Anti-Helicobacter pylori Activity
• Expression of chlorophyll a oxygenase and accumulation of chlorophyll b in cyanobacteria
• Chloroplast NAD Kinase is Essential for Energy Transduction Through the Xanthophyll Cycle in Photosynthesis
• Identification of a Novel Vinyl Reductase Gene Essential for the Biosynthesis of Monovinyl Chlorophyll in Synechocystis sp. PCC6803
• クロロフィルの生合成
• クロロフィル代謝
• クロロフィル代謝と植物の発育
• Identification of Chlorophyllide a Oxygenase in the Prochlorococcus Genome by a Comparative Genomic Approach
• 光合成光エネルギー捕捉装置の調節
• 再現実験から光合成の進化を考える (特集2 光合成の進化--植物の進化の原点に迫る)
• 光合成の起源と進化 光合成色素系の多様化の機構を探る
• クロロフィルa/b比はいかに制御されるのか 相互転換の解析から'クロロフィルサイクル'による調節の様相が判明
• Novel Amidine Conjugates of the Ornithine Moiety of the Macrocyclic Antifungal Lipopeptidolactone FR901469
• Site-specific Structural Transformation of the Novel Antifungal Cyclic Depsipeptide FR901469 : Synthesis and Biological Activity of FR203904
• The Major Route for Chlorophyll Synthesis Includes [3,8-divinyl]-chlorophyllide a Reduction in Arabidopsis thaliana
• Functional analysis of N-terminal domains of Arabidopsis chlorophyllide a oxygenase
• 葉緑体の起源と進化
• 緑色植物のクロロフィルb合成遺伝子の単離とその分子系統学的解析-原核緑藻類の進化-
• Photosynthetic Research in Plant Science
• Deregulated Chlorophyll b Synthesis Reduces the Energy Transfer Rate Between Photosynthetic Pigments and Induces Photodamage in Arabidopsis thaliana
• Light-Independent Cell Death Induced by Accumulation of Pheophorbide a in Arabidopsis thaliana
• The Arabidopsis-accelerated cell death gene ACD1 is involved in oxygenation of pheophorbide a: inhibition of the pheophorbide a oxygenase activity does not lead to the "stay-green" phenotype in Arabidopsis
• 地球環境と光合成の共進化
• Stoichiometric Analysis of Barley Plastid Ribosomal Proteins
• Knock-out of the Plastid Ribosomal Protein S21 Causes Impaired Photosynthesis and Sugar-Response during Germination and Seedling Development in Arabidopsis thaliana
• Ribosomal Proteins in the Cyanobacterium Anabaena variabilis Strain M3 : Presence of L25 Protein
• Overproduction of Chl b Retards Senescence Through Transcriptional Reprogramming in Arabidopsis
• The Oligomeric States of the Photosystems and the Light-Harvesting Complexes in the Chl b-Less Mutant
• クロロフィル合成系の多様性はいかにして生まれたか? (解説特集 光合成を支えるテトラピロール代謝の多様性)
• PS-163-8 当院において遺伝性乳癌が疑われた患者に行ったBRCA遺伝子診断に対する検討(PS-163 乳腺 予後因子,ポスターセッション,第112回日本外科学会定期学術集会)

もっと見る 閉じる

スポンサーリンク