Specific Increase in Phosphatase Isozymes in Cucumber Roots Caused by Calcium Deficiency

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Phosphatases in cucumber roots, whose production was induced by Ca^<2+> deficiency, were characterized chromatographically using ATP, 2'(3')-AMP and p-nitrophenyl-phosphate (PNPP) as substrates. Ca^<2+> deficiency stimulated greater than 10-fold in-creases in the activities with these substrates of the non-adsorbed fraction from a DEAE-cellulose column. Several fractions associated with these phosphatase activities were eluted from the column with NaCl solution; their levels increased less with Ca^<2+> starvation. When the non-adsorbed fraction from Ca^<2+>-starved roots was applied to a Sephadex G-200 column, fractions associated with 2'(3')-AMPase (phosphatase I) and with both ATPase and PNPPase (phosphatase II) were separated. In the control roots, very weak activities of phosphatases I and 11 were observed at the same position on the gel filtration. The phosphatase I isolated from both the control and Ca^<2+>_starved roots was extremely specific to nucleoside 2'(3')-monophosphates, whereas phosphatase II from both types of roots had a relatively broad substrate specificity. When phosphatase I from Ca^<2+>_starved roots was stained with 2'(3')-AMP in CaCl_2 after polyacrylamide gel electrophoresis, a single band was obtained. Phosphatase I from control roots also showed a single band, with the same Rf value. Phosphatase 11 from both types of roots contained two isozyme bands when the activities were stained with either ATP or PNPP. These results indicate that Ca^<2+> starvation causes specific increases in the level of phosphatases I and II in cucumber roots.
日本植物生理学会の論文

Specific Increase in Phosphatase Isozymes in Cucumber Roots Caused by Calcium Deficiency

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