Reduced Stability of Chromatin Structure of Pea Roots Grown under Calcium Starvation
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The growth of pea seedlings was reduced markedly when Ca^<2+> was omitted from the culture medium. Some properties of chromatin from pea roots cultured with a complete supply of nutrients or a medium lacking Ca^<2+> were compared. Tm of the control chromatin from Ca^<2+>-supplied plants was higher than that of the chromatin from Ca^<2+>-deficient plants. Addition of EGTA or EDTA to control chromatin shifted its Tm to a lower temperature concomitant with an increase of hyperchromicity. The chromatin prepared from Ca^<2+>_deficient plants was more susceptible to DNase I and II digestion. When solubilized chromatin was digested with a certain amount of DNase II and chromatographed with Bio-Gel A5m column, it turned out that digestion of the chromatin from Ca^<2+>-deficient plants progressed more than that of the control chromatin. Template activity of the chromatin prepared from Ca^<2+>-deficient plants was higher than that of the control chromatin, suggesting that unspecific transcription might be induced by relaxation of the chromatin structure due to Ca^<2+> deficiency. The Ca^<2+> contents of nuclear fractions were considerably lower in the Ca^<2+>-deficient roots than in the control roots. The results suggest that differences in Ca^<2+> nutrition can alter chromatin structure.
- 日本植物生理学会の論文
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