OCCURRENCE OF THREE MACROMOLECULAR INHIBITORS OF NITRATE REDUCTASE IN CHINESE CABBAGE ROOTS

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Three macromolecular inhibitors of nitrate reductase were isolated from an extract of chinese cabbage roots by DEAE=cellulose column chromatography. These inhibitors, I, II, and III, had an inhibitory effect on NADH-nitrate reductase activity but had no effect on the activity of nitrite reductase and NADH-glutamate dehydrogenase from chinese cabbage leaves. Inhibitor I and III also strongly inhibited the activity of FMNH_2-nitrate reductase and only slightly that of glutamine synthetase, whereas inhibitor II had a weaker inhibitory effect on FMNH_2-nitrate reductase activity and no effect on glutamine synthetase activity. Although these inhibitors were heat-labile, inhibitor II was relatively less sensitive to heat treatment compared to both inhibitors I and III. Inhibitory effect of I, II and III was caused by neither the presence of phenolic compounds or corresponding quinones nor the oxidation of NADH which is required for nitrate reductase activity. Preincubation of the inhibitors with enzyme was required for achieving maximum inhibition of nitrate reductase activity. Furthermore, the reaction(s) taking place during the preincubation period was thermo-dependent. High activities of protease and peroxidase were detected in the fraction inhibitor I. It seemed that the inhibition was not caused by the rapid binding of the inhibitors to the enzyme, and that proteolytic activity played a role in the inhibitory activity of at least inhibitor I.
社団法人日本土壌肥料学会の論文

OCCURRENCE OF THREE MACROMOLECULAR INHIBITORS OF NITRATE REDUCTASE IN CHINESE CABBAGE ROOTS

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