SOME REGULATIVE PROPERTIES OF GLUTAMINE SYNTHETASE IN CUCUMBER LEAVES
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概要
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Glutamine synthetase (L-glutamate : ammonia ligase (ADP) EC 6.3.1.2) was prepared from cucumber leaves grown on ammonium medium and some regulative properties were investigated. The apparent Michaelis constant (Km) values of the various substrates and cofactors were determined. Lineweaver-Burk plots gave a Km of 4.5 mM with L-glutamate, 0.74mM with ATP and 3.1 mM with NH_2OH. Metals, including Cu^<2+>, Hg^<2+>, Zn^<2+>, Ni^<2+>, Fe^<2+>, and Fe^<3+>, strongly inhibited the enzyme activity. Cd^<2+> did not inhibit the enzyme activity markedly, in contrast with its effect on the rice enzyme. Ca^<2+> was quite inhibitive to enzyme reaction and more than 50% of the activity was lost at 3 mM. The amino acids tested generally had no effect on the enzyme activity except alanine, which showed little but clear inhibition. Isocitrate and α-ketoglutarate were slightly promotive to enzyme activitv while pyruvate and glyoxylate (24 mM) significantly inhibited the enzyme activity. Glucose-1- or -6-phosphate and fructose-6-phosphate were inhibitive to similar degrees, about 20% at 22.5 mM, and 3-phosphoglycerate (22.5 mM) markedly inhibited the enzyme activity up to 56%. Among the nucleotides tested, UTP, CTP, and GTP inhibited slightly, and marked inhibitions of 44 and 78% were observed after the addition of AMP (5 mM) and ADP (5 mM) respectively. Pyridoxal-5'-phosphate, which is a characteristic incompetitive inhibitor of L- glutamate and NH_2OH, here inhibited enzyme activity significantly. On the other hand, pyridoxamine-5'-phosphaie had almost no effect. The inhibition caused by the former was not recovered by the latter. This response of glutamine synthetase to both compounds was in agreement with the idea that the nitrogen status of the plant could be reflected by the ratio pyridoxamine phosphate/pyridoxal phosphate. Also this suggested that the regulative properties of glutamine synthetase with respect to pyridoxal or pyridoxamine phosphate could be understood from the point of view of the economical use of nitrogen.
- 社団法人日本土壌肥料学会の論文
著者
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Takahashi Eiichi
Department Of Agricultural Chemistry. Kyoto University
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Matsumoto Hideaki
Institutefor Agricultural And Biological Science Okayama University
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Hirasawa Eiji
Department Of Biology. Faculty Of Science Osaka City University
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Hirasawa Eiji
Department Of Agricultural Chemistry Kyoto University
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Matsumoto Hideaki
Department Of Agricultural Chemistry Kyoto University
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KAWANO Sumiko
Department of Agricultural Chemistry, Kyoto University
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Takahashi Eiichi
Department Of Agricultural Chemistry Kyoto University
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Matsumoto Hideaki
Department Of Agirucltural Chemistry Kyoto University
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Takahashi Eiichi
Department Of Agirucltural Chemistry Kyoto University
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Kawano Sumiko
Department Of Agricultural Chemistry Kyoto University
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