ブタ副腎ミクロソームにおける21-ヒドロキシラーゼおよび17α-ヒドロキシラーゼ活性の一酸化炭素による阻害

概要

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21-Hydroxylase, 17α-hydroxylase and C_<17-20> lyase activites on porcine adrenal microsomes were measured by incubation of radioactive steroids with nicotineamide adenine dinucleotide phosphate. [4-^<14>C]-Progesterone, [4-^<14>C]-pregnenolone, [4-^<14>C]-17α-hydroxyprogesterone and [4-^<14>C]-11-deoxycorticosterone were used as a substrate. 21-Hydroxylase and 17α-hydroxylase activities showed the maximum value in 170 mM and 80 mM potassium phosphate buffer (pH 7.5), respectively. Inhibitory effect of carbon monoxide on 21-hydroxylation and 17α-hydroxylation of progesterone by porcine adrenal microsomes was investigated. The degree of inhibition on two hydroxylation reactions differed. The rate of CO to O_2 needed for 50% inhibition of the 21-hydroxylase and 17α-hydroxylase activities were 3.22 and 29.1,respectively.
公益社団法人日本薬学会の論文
1983-08-25