ブタ副腎ミクロソーム画分より3β-ヒドロキシステロイドテヒドロゲナーゼ/イソメラーゼの精製と酵素活性に及ぼす膜構造形態変化の影響

概要

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A 3β-hydroxysteroid dehydrogenase/Δ^4-Δ^b-isomerase (3β-HSD/isomerase) has been purified to homogeneity from the pig adrenal microsomes by solubilization with sodium cholate, followed by some conventional column chromatographies. The molecular weight of the enzyme was estimated to be 42000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The K_m value for pregnenolone as the substrate of the purified enzyme was higher than that of the microsomal enzyme. The purified enzyme was less stable for heat treatment than the microsomal binding enzyme. The microsomal enzyme was treated with various agents or enzymes which destroyed the membrane structure. Lipid peroxidation with Fe^<2+>, digestion with phospholipase A_2 or C, and the addition of various free fatty acids, imidazole containing compounds and polymyxin B were utilized for the membrane destruction. Consequently, the 3β-HSD/isomerase activity was significantly reduced in all cases, and the K_m value of the 3β-HSD/ isomerase for pregnenolone increased. And the V_<max> and V_<max>/K_m values significantly decreased. Therefore, it is strongly suggested that the normal membrane structure plays an important role in the maintenance of the 3β-HSD/isomerase activity.
公益社団法人日本薬学会の論文
1993-03-25