チトクロームb_5を伴ったチトクロームP-450(17α-ヒドロキシラーゼ/リアーゼ)酸素添加酵素系の触媒するΔ^<16>-C_<19>-ステロイド合成活性

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In order to clarify the effect of cytochrome b_5 on Δ^<16>-C_<19>-steroid synthesizing activity in the testicular microsomes of neonatal pig, the effects of antibody and pyridine nucleotide on cytochrome b_5 were investigated. Furthermore, the relationship between Δ^<16>-C_<19>-steroid synthesizing activity and cytochrome b_5 was studied by use of adrenal microsomes or a reconstituted enzyme system of cytochrome P-450 (17α-hydroxylase/lyase) purified from adrenal microsomes. With anti-cytochrome b_5 IgG no remarkable change was observed in the 17α-hydroxylase/lyase activity of testicular microsomes. Δ^<16>-C_<19>-Steroid synthesizing activity was, however, strongly inhibited. In the testicular microsomes or the reconstituted enzyme system of cytochrome P-450 (17α-hydroxylase/lyase) isolated from testicular microsomes, synergetic effects of nicotinamide adenine dinucleotide phosphate and nicotinamide adenine dinucleotide against the Δ^<16>-C-_<19>-steroid synthesizing activity and 17α-hydroxylase/lyase activity was observed. A strong effect was detected on the Δ^<16>-C_<19>-steroid synthesizing activity, while this synergetic effect against 17α-hydroxylase/lyase activity was weak. Although in adrenal microsomes little Δ^<16>-C_<19>-steroid synthesizing activity was detected, this activity was restored by the addition of cytochrome b_5 isolated from testicular microsomes. Similarly to microsomes, Δ^<16>-C_<19>-steroid activity was restored by the addition of cytochrome b_5 to the reconstituted enzyme system of cytochrome P-450 (17-hydroxylase/lyase) isolated from adrenal microsomes.
公益社団法人日本薬学会の論文
1987-02-25

チトクロームb_5を伴ったチトクロームP-450(17α-ヒドロキシラーゼ/リアーゼ)酸素添加酵素系の触媒するΔ^<16>-C_<19>-ステロイド合成活性

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