テヒドロ酢酸およびそのナトリウム塩のホウ酸の無水酢酸溶液によるケイ光定量

概要

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For the determination of dehydroacetic acid or its sodium salt, the fluorescence reaction of aromatic o-hydroxycarbonyl compounds with boric acid reported previosly was successfully applied. A solution (9.0ml), 2-100ng/ml of dehydroacetic acid or its sodium salt in a mixture of equal volumes of acetic acid and acetic anhydride was mixed with 1.0ml of 6% w/v solution of boric acid in acetic anhydride in a stoppered test tube and was kept for 30 min at 45°. The relative intensity of fluorerscence of the solution was measured. The maximum wave length of the corrected excitation spectrum of the fluorescent solution was 330 mμ and that of the corrected emission spectrum was 362 mμ. The calibration cruve was linear in the range of 1-1000ng/ml of dehydroacetic acid or its sodium salt. This method was not interfered by the presence of various compounds except a large amount of AF-II, salicylic acid, and ferric chloride. Sodium dehydroacetate in butter was almost quantitatively determined by this method without any pretreatment other than extraction.
公益社団法人日本薬学会の論文
1968-05-25