蛋白分解酵素処理モルモット摘出腸管のBradykinin, Histamine, Acetylcholineに対する感受性の変化

概要

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Bromelain in a concentration of 0.1 mg/ml, likewise chymotrypsin, trypsin, and papain, potentiated the response of isolated guinea-pig ileum to bradykinin, but not to acetylcholine and histamine. In higher concentration of the enzymes, however, the contraction induced by bradykinin, acetylcholine and histamine was depressed. The intensity of this potentiation or depression effect was correlated with the caseinolytic activities of the proteolytic enzymes. This phenomenon was not found in the case of compounds with a large molecule such as DNA, casein, and egg albumin. Heat denaturation of the proteolytic enzymes eliminated their potentiation or depression effect. Even if the proteolytic activity of bromelain or papain was completely inhibited by p-chloromercuribenzoate, this potentiation or depression effect was still present. These inactivated enzymes were not reactivated by incubation with guinea-pig ileum. From these results, possible mechanism of this effect was discussed.
公益社団法人日本薬学会の論文
1968-12-25