ヒト前立腺酸性ホスファターゼに関する研究(第1報)各種基質の加水分解におよぼす界面活性剤およびウシ血清アルブミンの影響

概要

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Prostatic acid phosphatase was purified from human prostate gland by ammonium sulfate fractionation, phosphate-cellulose, Sephadex G-100,and DEAE-cellulose column chromatography. The purified enzyme was free of human serum protein and had a single protein corresponding to phosphatase activity by disc electrophoresis. Effect of various detergents and bovine serum albumin on hydrolysis of six substrates by purified prostatic acid phosphatase was investigated. Enhancing effect of various nonionic detergents and bovine serum albumin on hydrolysis of thymolphthalein monophosphate was observed. Optimal concentration of the enhancing effect was observed. In less than the optimal concentration, Lineweaver-Burk plots presented increase of K_m and V_<max> values, while in more than the optimal concentration K_m value increased without change in V_<max> value.
公益社団法人日本薬学会の論文
1978-09-25