Bromazepamの代謝に関する研究(第4報)2-(2-Amino-5-bromobenzoyl)-pyridineの肝ミクロゾームによる水酸化

概要

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Hydroxylation of 2-(2-amino-5-bromobenzoyl) pyridine (ABBP) to 3-OH-ABBP in animal liver was found to be mediated by the microsomal mixed-function oxidase system requiring nicotinamide adenine dinucleotide phosphate reduced form (NADPH) and O_2. When ABBP was incubated with microsomal fraction prepared from the liver of rats treated with phenobarbital or benzo [α] pyrene, hydroxylation of ABBP was enhanced about 2-fold of that of control rats. Hydroxylation of ABBP by microsomes of rats and rabbits was inhibited by SKF-525A, HgCl_2,CuCl_2,p-chloromercuribenzoate (PCMB), cytochrome c, and CO. The kinetic constants of ABBP hydroxylation by liver microsomes of various animal species indicated a similar affinity of ABBP. It is of interest that a factor which activates the hydroxylating activity was found in the soluble fraction of various organs of animals.
公益社団法人日本薬学会の論文
1974-08-25