チオールおよびジスルフィド化合物の研究(第2報)分子中にアミノ基をもたないチオール化合物の血中レベルの定量法とその種物質の一つとしての2-メルカプトプロピオニルグリシンの吸収, 排泄研究への応用

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A simple method was established for the determination of some amino group-free thiol and disulfide (AGF-SH, S-S) compounds in blood. Absorption and excretion were studied by giving 2-mercaptopropionylglycine (MPG), one of AGF-SH compounds, to rats. In addition, absorption was compared among some thiol compounds. 1. All the non-protein thiols and disulfides (NPSH, S-S) in blood could be removed by passing the deproteinized solution through a column of sulfonated ion-exchange resin as they possess amino groups in their molecule. This made it possible to determine AGF-SH, S-S compound that appeared in blood. 2. Blood AGF-SH level reached its maximum 1 hr after oral administration of 100 mg/kg MPG. AGFS-S compound also appeared in blood, and 23% of a given MPG was excreted in urine by 5 hr and urinary mercaptopropionic acid, metabolite of MPG, was less than 2% of a given dose. 3. Absorption was compared among some thiol compounds by estimation of NPSH, S-S in plasma after their administration. Intraperitoneal administration of MPG and glutathion (GSH) led to a remarkable increase of NPSH and NPS-S in plasma after 15 min, but administration of 2-(S-benzoyl)mercaptopropionylglycine (S-benzoyl MPG) resulted in a slight increase of them. Oral administration of MPG, S-bemzoyl MPG and cysteine led to an increase of NPSH in plasma after 1 hr, but administration of GSH did not.
公益社団法人日本薬学会の論文
1973-05-25