液体シンチレーション計数装置による生体試料中の^<14>Cの測定法, 湿式酸化による測定試料の小型調製装置

概要

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The apparatus used for measuring carbon-14 in a biological sample by wet combustion method was improved. In order to treat a few samples at the same time and to expel the evolved ^<14>CO_2 promptly, the decomposition flask was made as small as possible. A biological material, usually an organ itself or its homogenate equivalent to about 100 mg of wet sample, was placed in the decomposition vessel, and 15 ml of oxidizing agent, prepared from chromic trioxide (25 g), potassium iodate (5 g), phosphoric acid (d 1.7,167 ml), and 60% fuming sulfuric acid (333 ml), was added by turning the reservoir 180°. The vessel was heated at 180-200° for 1 hr. ^<14>CO_2 was expelled with nitrogen and bubbled through a scintillator containing ethanolamine. This method was applicable not only to biological materials but also to such an insoluble sample as a resin. Recovery of carbon-14 was 98-100%. Scatter of measured values was examined with the same liver homogenate of a rat administered l-ephedrine[α-^<14>C] and its standard deviation was 2.1%.
公益社団法人日本薬学会の論文
1973-04-25