発ガン性アゾ色素の研究(第4報)ラット, マウスおよびハムスター肝による3'-Methyl-4-dimethylaminoazobenzene(5'-^3H)の代謝反応における種差に関する研究

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The species differences in the metabolism of the carcinogenic azo dye, 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB), by the liver of rats, mice, and hamsters were studied by the tracer method. 3'-Me-DAB[5'-^3H] was incubated with the liver homogenate of each experimental animal, and 72-75% of the radioactivity added was recovered in the benzene-acetone (1 : 1) layer from the incubation mixture. The substrate and metabolites in the extracts were separated and isolated by preparative thin-layer chromatography on silica gel plates and identified by the reverse isotope dilution analysis. The overall detoxication activity of this carcinogen in mouse liver was the highest and that of hamster liver was similar to that of rat liver. In these normal livers the azo-reduction was the main ditoxicative metabolism of 3'-Me-DAB, and the azo-reduction activity of hamster liver was the lowest, while the actvvity of mouse or rat liver was about two or three times greater.
公益社団法人日本薬学会の論文
1973-12-25