液体シンチレーション計数装置による生体試料中の^<14>Cの測定法および^3H, ^<14>Cの同時測定法

概要

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Methods were examined for measuring ^<14>C and for simultaneous measuring of ^3H and ^<14>C in biological samples. A lyophilized organ material was pulverized and about 20 mg of the powder was burnt in an oxygen flask. Scintillator containing ethanolamine was used for radioactive carbon dixoide and a scintillator containg dnioxan and ethanolamine was used for simultaneous measurement of ^3H and ^<14>C. Recovery of ^<14>C was 96.3% and its standard deviation was 1.4%. Reproducibility of the assay was further examined with the same liver of a rat given ^<14>C-ephedrine with satisfactory results, i.e. the standard deviation was 0.8%. Recoveries of ^3H and ^<14>C were 99.8 and 99.3% respectively in the simultaneous measurement, and their standard deviation was 1.3 and 1.5%.
公益社団法人日本薬学会の論文
1968-05-25