カラム抽出高速液体クロマトグラフィーによる尿中覚せい剤の定量

概要

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A method for the quantitative analysis of sympathomimetic amines, methamphetamine (MA) and amphetamine (A), in the urine was developed by using column extraction-high-performance liquid chromatography (HPLC). Simple and rapid extraction of MA and A from the urine samples was carried out by using Bond Elut^[○!R] pre-packed column with acidified acetonitrile aqueous solution. The mean extraction ratio was 94.9% with the coefficient of variation of 1.66% (n=5). The HPLC was performed by utilizing a reversed-phase column with 0.05M ammonium acetate and 0.2% acetic acid in 50% acetonitrile and by monitoring at 254 nm. The sympathomimetic amines were eluted at a flow rate of 1.0 ml/min and the quantity of MA was estimated from the peak height. This method facilitated MA determination within 1.69% error over the urine level range from 5 to 100 μg/ml, with the limit of detection being 1 μg/ml. After intravenous injection of 2 mg MA in rats, the mean amount of MA excreted after 24 h in the urine was about 25% of the dose administered. The method was successfully applied to human urine samples containing MA.
1984-02-29