化学発光検出高速液体クロマトグラフィを用いるヒト尿中覚せい剤分析法

概要

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High-performance liquid chromatographic method has been developed for the determination of trace levels of methamphetamine, amphetamine and piperidine in the human urine. Three compounds extracted into diethyl ether from alkaline urine were derivatized with dansyl chloride, and separated on a reversed-phase column and detected chemilumigenicaly by using bis (2,4,6-trichlorophenyl) oxalate and hydrogen peroxide as post column reagents. Methamphetamine as low as 2×10^<-10>M level in the urine was determined by the proposed method. The sensitivity of the proposed method was higher than that of gas-chromatography with flame ionization detection (FID-GC). The analytical results of methamphetamine in the urine samples of human who took methamphetamine by i.v. injection were in good agreement with those by FID-GC.
公益社団法人日本薬学会の論文
1991-02-28