フナ肝臓中に誘導されるメタロチオネインの性質

概要

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The low molecular weight cadmium-binding protein induced in the liver of the fish (giber ; Carassius auratus langsdorfi) by cadmium loading was shown to be a mixture of two isoproteins. The two isoproteins were referred to gibel metallothionein-I and -II (MT-I and -II) according to the order of elution from a DEAE Sephadex column. The molecular weight of the purified metallothioneins was estimated to be similar to that of mammalian metallothioneins by amino acid analyses. Metals in the gibel metallothioneins were mainly cadmium (around 90 mol%), and zinc and copper were present as minor co-existing metals. The SH/metal ratios were 2.9 (MT-I) and 2.2 (MT-II) Amino acid analyses revealed characteristic amino acid compositions which were consistent with the metallothionein concepts ; high cysteine contents (36.8 and 38.2 mol%), without aromatic amino acids and histidine. As relatively abundant amino acid residues in the gibel metallothioneins, lysine, serine and glysine (9.1-11.6 mol%) were present other than cysteine residue. Both of the isoproteins contained a single methionine as in the case of mammalian metallothioneins.
公益社団法人日本薬学会の論文
1983-04-30