ラットに気管内投与した重金属の影響(第4報)肺における酢酸亜鉛の代謝

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Zinc acetate was instilled intratracheally into rats and the metabolic fate of zinc ions in the lung was examined. Male Wistar rats (14 weeks old, mean body weight 371 g) were instilled at a dose of 20μg Zn/0.4 ml saline/rat and killed 1/6,1/2,1,3,6 and 12 h and 1,2,3 and 7 d for the time-course experiment, and the animals were instilled at doses of 5,10,20,50,100 and 200 μg/rat and killed 2 d later for the dose-dependent experiment. Concentrations of zinc and other elements in the lungs, livers, pancreases, kidneys, supernatants of lungs and broncho-alveolar lavage fluids were determined by inductively coupled argon plasma-atomic emission spectrometry (ICP). Zinc instilled in the lung was shown to be taken up more than 90% by the lung within 3 h. However, the zinc taken up by the lung was transferred immediately to the liver, pancreas and other organs. A portion of the zinc taken up by the lung was present in the supernatant fraction of the lung and the zinc in this fraction disappeared more slowly than that in the whole lung. Distribution profiles of zinc and other elements in the supernatant of the lung were determined by the HPLC-ICP method. Only copper profiles showed a significant change. Zinc was not found in the metallothionein fraction, while copper increased in this fraction with doses of zinc instilled into the lung.
社団法人日本薬学会の論文
1988-12-31