尿中第四級アンモニウム塩系薬物のサーモスプレイLC-MSによる分析

概要

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A simple and rapid method for the detection of 12 quaternary ammonium compounds (Suxamethonium, Pancuronium, Benzethonium, Acetylcholine, Bethanechol, Hexamethonium, Propantheline, Methylbenactyzium, Neostigmine, Distigmine, Pyridostigmine and Ambenonium) using thermospray high-performance liquid chromatography (LC)-mass spectrometry was investigated. Each compound was extracted from urine as an ion-pair with bromothymol blue, tropaeolin OO and hexanitrodiphenilamine in dichloromethane. The used LC column was Asahipak GS-320H for aqueous steric exclusion chromatography, and the mobile phase was 100 mM ammonium acetate-acetonitrile (90/10 v/v or 70/30 v/v). The ion source block and tip heater temperatures were set at 300 and 320℃, respectively. The vaporizer control temperature was kept at the temperature of the take off temperature minus 15℃. The recoveries of 12 quaternary ammonium compounds from the spiked urine were 76.1 to 98.6% by ion-pair extraction. The detection limits of these compounds for the simultaneous analysis were 0.5-80 ng/ml by selected ion monitoring mode.
公益社団法人日本薬学会の論文
1994-12-31