Determination of Dibucaine and Its Metabolites in Human Urine by High-Performance Liquid Chromatography with Fluorescence Detector(Analytical,Chemical)

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A high-performance liquid chromatographic(HPLC) method using a fluorescence detector is described for the simultaneous determination of dibucaine and its metabolites(M-4, M-8 and M-10) in human urine. Urine samples from obstetric patients were chromatographed in a re versed-phase system. When a ultraviolet detector(320 nm) was used, some interfering peaks appeared on the chromatogram, but this interference could be overcome by employing a fluorescence detector(Ex 330nm and Em 440nm) instead. The calibration curves were linear in the range of 0.05-5.0μg/ml for all compounds and the detection limits of dibucaine and its metabolites were about 5ng/ml in urine. The urinary excretion of dibucaine and its metabolites by obstetric patients infused with Percamine S^[○!R] in the spinal cord were determined. The mean cumulative amounts of dihucaine, M-4, M-8 and M-10 excreted during 10 h after administration were 1.1,10.5, 3.5 and 1.1% of the dose, respectively. The total urinary excretion was 16.2% of the dose. This method is sufficiently sensitive and specific to permit the determination of dibucaine and its metabolites in biological fluids.
公益社団法人日本薬学会の論文
1987-07-25