熱分解ガスクロマトグラフィーの裁判化学への応用(第1報)アミノ酸及びタンパク質試料の分析

概要

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In order to carry out a rapid analysis of amino acids, proteins and related materials without troublesome pretreatment, application of pyrolysis gas chromatography (PGC) for these samples was investigated. Highly resolved pyrograms were obtained from these materials by using Curie point pyrolyser and fused-silica capillary column with moderate polarity. At first, PGC of 21 major L-α-amino acids was studied, and 18 of them were identified by using 1 μg of each sample. Secondly, PGC of protein samples, such as egg white lysozyme, wool fiber, etc. was examined. The major peaks observed on these pyrograms were well assigned, and it was possible to discriminate trace protein samples by comparison with the differences in the amino acid residues.
公益社団法人日本薬学会の論文
1988-10-31