Properties of Cationic Liposomes Composed of Cationic Lipid YKS-220 Having an Ester Linkage : Adequate Stability, High Transfection Efficiency, and Low Cytotoxicity

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概要

• 論文の詳細を見る

• Cationic lipid N-[3-[2-(1,3-dioleoyloxy)propoxy-carbonyl]propyl]-N, N, N-trimethylammonium iodide (YKS-220) having a symmetrical and biodegradable structure was employed for the preparation of cationic liposomes with dioleoylphosphatidylethanolamine (DOPE). The stability, transfection activity in several cell lines and cytotoxicity of YKS-220 cationic liposomes were studied. It was found the YKS-220 cationic liposomes were very stable and their transfection activity remained even after storage at 4℃ for 12 months. The transfection activity of these liposomes was assayed using CHO, COS, and HepG2 cells and found to be comparable with, or better than, that of other cationic liposomes, such as N-[1-(2,3-dioleoyloxy)propyl]-N, N, N-trimethylammonium methylsulfate (DOTAP) liposome, N-[1-(2,3-dioleyloxy)propyl]-N, N, N-trimethylammonium chloride (DOTMA) liposome (Lipofectin^<[○!R]>), and 2,3-dioleyloxy-N-[2-(sperminecarboxamido)ethyl]-N, N-dimethyl-1-propanaminium trifluoroacetate (DOSPA) liposome (LipofectAMINE^<TM>). n addition, the cytotoxicity of YKS-220 cationic liposomes was far lower than that of other cationic liposomes.

• 公益社団法人日本薬学会の論文
• 1999-02-15

著者

• 今西 武

  大阪大学大学院薬学研究科

• 高橋 以都美

  Faculty Of Pharmaceutical Sciences Osaka University

• DOI Takefumi

  Graduate School of Pharmaceutical Sciences, Osaka University

• Yu Wie

  Graduate Shool Of Pharmaceutical Sciences Osaka University

• Yu W

  Shock Wave And Condensed Matter Research Center Kumamoto University

• Obika Satoshi

  Graduate School of Pharmaceutical Sciences, Osaka University

• Imanishi Takeshi

  Graduate School of Pharmaceutical Sciences, Osaka University

• Imanishi Takeshi

  Graduate Shool Of Pharmaceutical Sciences Osaka University

• Imanishi Takeshi

  Graduate School Of Pharmaceutical Sciences Osaka University

• Doi Takefumi

  Graduate School Of Pharmaceutical Sciences Osaka University

• Doi Takefumi

  Graduate Shool Of Pharmaceutical Sciences Osaka University

• Obika Satoshi

  Graduate School Of Pharmaceutical Sciences Osaka University

• Obika Satoshi

  Faculty Of Pharmaceutical Sciences Osaka University

• Minami T

  Department Of Science And Technology Kinki University

• Imamura T

  Faculty Of Pharmaceutical Sciences Osaka University

• YU Wei

  Graduate Shool of Pharmaceutical Sciences, Osaka University

• SHIMOYAMA Atsuko

  Graduate Shool of Pharmaceutical Sciences, Osaka University

• UNEDA Takeshi

  Graduate Shool of Pharmaceutical Sciences, Osaka University

• MIYASHITA Kazuyuki

  Graduate Shool of Pharmaceutical Sciences, Osaka University

• MINAMI Takeshi

  Graduate School of Pharmaceutical Sciences, Osaka University

• Uneda Takeshi

  Graduate Shool Of Pharmaceutical Sciences Osaka University

• 小比賀 聡

  Osaka Univ. Osaka Jpn

• Miyashita K

  Graduate Shool Of Pharmaceutical Sciences Osaka University

• Shimoyama Atsuko

  Graduate Shool Of Pharmaceutical Sciences Osaka University

• Minami Takeshi

  Graduate School Of Nutritional And Environmental Sciences And Center Of Excellence For The 21^<st

• Minami Takeshi

  Graduate School Of Pharmaceutical Sciences Osaka University

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