Generation of Immortalized Murine Forebrain Cell Lines Expressing an α Isoform of Ca^<2+>/Calmodulin-Dependent Protein Kinase II
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概要
- 論文の詳細を見る
Immortalized hybrid cells were generated by the somatic fusion of the cells from the forebrain of embryonic mouse with N18TG2 neuroblastoma cells. Three monoclonal hybrid cell lines, designated NF26,NF81,and NF83 (n__-euroblastoma f__-orebrain hybrid cells), expressing an α isoform of Ca^<2+>/calmodulin-dependent protein kinase II (CaM kinase II) were isolated, and their expression was demonstrated by immunoblotting and immunocyto-chemistry using a monoclonal antibody specific to the α isoform of the enzyme. The kinase activity of the hybrid cells was 2- to 3-fold higher than that of the parent neuroblastoma line N18TG2 cells. The neuronal origin of these lines was shown by their immunoreactivity to neurofilament protein, a neuron specific marker. Lines NF26,NF81,and NF83 are the first cell lines to express the gene of the α isoform of CaM kinase II in the brain.
- 公益社団法人日本薬学会の論文
- 1998-03-15
著者
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YAMAUCHI Takashi
Department of Industrial Chemistry, Faculty of Engineering
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Yamazaki Tadashi
東京大学 医学系研究科循環器内科
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YOSHIMURA YOSHIYUKI
Department of Biochemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima
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Yoshimura Y
Department Of Analytical Chemistry Hoshi University
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Yamauchi T
Faculty Of Medicinal Chemistry; Hoshi University
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FUJII Michiho
Department of Biochemistry, Faculty of Pharmaceutical Sciences, University of Tokushima
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NOMURA Tomohiko
(Present address)Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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KUMATORIYA Kyoko
Department of Biochemistry, Faculty of Pharmaceutical Sciences, University of Tokushima
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Yamauchi T
Dep. Of Biochemistry Inst. Of Health Biosciences And Graduate School Of Pharmaceutical Sciences The
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Yamauchi Takashi
Department Of Bioresources Sciences Faculty Of Agriculture Kyushu University
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Yoshimura Y
Department Of Biochemistry Faculty Of Pharmaceutical Sciences University Of Tokushima
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Yoshimura Yoshiyuki
Department Of Biochemistry Faculty Of Pharmaceutical Sciences The University Of Tokushima
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Fujii Michiho
Department Of Biochemistry Faculty Of Pharmaceutical Sciences University Of Tokushima
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Kumatoriya Kyoko
Department Of Biochemistry Faculty Of Pharmaceutical Sciences University Of Tokushima
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Yamauchi Takashi
Department Of Applied Physics Faculty Of Engineering Osaka University
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