^1H-Nuclear Magnetic Resonance Studies on Interaction between 4-[4-(N, N-Dimethylcarbamoylme- thoxycarbonymethyl)phenoxycarbonylphenyl]guanidinium Methanesulfonate and Trypsin

概要

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The 600 MHz ^1H-nuclear magnetic resonance spectra of a mixed aqueous solution of typsin and a synthesized trypsin inhibitor, 4-[4-(N, N-dimethylcarbamoylmethoxycarbonylmethyl)phenoxycarbonylphenyl]-guanidinium methanesulfonate(FOY-305) were measured. Signals assigned to the inhibitor protons indicated that the hydrated fragment of the inhibitor formed a complex with trypsin. The signal change during 4 h of incubation at 37℃ was interpreted as reflecting a successive process of the partial release of the fragment and its weak recombination with the enzyme around the active site. The result is consistent with the disorder of the fragment in the crystal structure of the inhibitor : trypsin complex.
社団法人日本薬学会の論文
1990-07-25