Enhancement of the Sensitivity of a Fluorometric Lysozyme Assay System by Adding β-N-Acetylhexosaminidase
スポンサーリンク
概要
- 論文の詳細を見る
The lysozyme assay using 4-methylumbelliferyl tetra-N-acetyl-β-chitotetraoside as a substrate was found to show increased sensitivity for lysozyme in the presence of jack bean β-N-acetylhexosaminidase (NAHase). The assay system containing NAHase gave a linear dose-response curve in the range of 10-70μg of lysozyme under conditions of 20-60 min incubation at pH 5.2 and 35℃, and the sensitivity for lysozyme was more than five times higher than that of the fluorometric assay system without the hexosaminidase. The new assay gave values compatible with those of the cell turbidimetric assay using Micrococcus lysodeikticus as a substrate in the estimation of lysozyme in pharmaceutical preparations. The present assay system has better reproducibility than the cell turbidimetric assay and may serve as a substitute for the latter.
- 社団法人日本薬学会の論文
- 1985-08-25
著者
-
山羽 力
National Institute of Hygienic Sciences
-
山羽 力
国立衛生試験所
-
谷本 剛
National Institute of Hygienic Sciences (NIHS)
-
福田 秀男
National Institute of Hygienic Sciences
-
福田 秀男
Division Of Biological Chemistry And Reference Standards National Institute Of Hygienic Sciences
-
谷本 剛
国立衛生試験所大阪支所薬品試験部
-
谷本 剛
Division Of Biological Chemistry And Biologicals National Institute Of Hygienic Sciences
-
谷本 剛
National Institute Of Hygienic Sciences
関連論文
- 酵素製剤の定量試験-2-ブロメライン製剤
- Purification and Characterization of the Product of Chemically Synthesized Human Growth Hormone Gene Expression in Escherichia coli(Biological,Chemical)
- PARTIAL PURIFICATION AND BIOLOGICAL ACTIVITY OF THE PRODUCT OF CHEMICALLY SYNTHESIZED HUMAN GROWTH HORMONE GENE EXPRESSION IN ESCHERICHIA COLI
- Studies on Lens-Aldose-Reductase Inhibitor in Medicinal Plants II. : Active Constituents of Monochasma savatierii FRANCH. et MAXIM.
- 高速液体クロマトグラフィーによるインシュリンの分析(第4報)塩酸溶液中のインシュリンの安定性
- Influence of the Structure of the A and B Rings of Various 20-Oxo-steroids on Their Interaction with 20β-Hydroxysteroid Dehydrogenase
- CHARACTERIZATION OF CONTAMINANTS IN EMS-ASSOCIATED L-TRYPTOPHAN SAMPLES BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
- Conversion of Cyclamate to Cyclohexylamine in Guinea Pig
- Excretion of Cyclohexylamine, a Metabolite of Cyclamate, in Human Urine
- ^C-標識ジフェニルの小規模合成とマウス肝ミクロソームへの結合
- 加硫促進剤,N-Cyclohexyl-2-benzothiazyl Sulfenamide(CBS)のラットによる吸収,分布,代謝および排泄
- Comparative Studies on Absorption, Distribution, and Excretion of Flame Retardants Halogenated Alkyl Phosphate in Rats
- Metabolism of Thiabendazole and Teratogenic Potential of Its Metabolites in Pregnant Mice(Environmental)
- Reaction and Inhibition Mechanisms of Aldose Reductase from Rabbit Lens
- Inhibitory Effect of Benzyl Oxazolecarbamate Analogues on Aldose Reductase
- Enhancement of the Sensitivity of a Fluorometric Lysozyme Assay System by Adding β-N-Acetylhexosaminidase
- 1,1'-Ethylene-bis(1-nitrosourea)(EBNU)の生化学的研究(第8報)EBNUおよび関連化合物によるラット肝臓クロマチンのカルバモイル化とその影響
- 1,1'-Ethylene-bis(1-nitrosourea)(EBNU)の生化学的研究(第7報)EBNUおよび関連化合物のラット肝臓クロマチンの鋳型活性に及ぼす影響
- Distribution of Butylated Hydroxyanisole and Its Conjugates in the Tissues of Rats
- Pseudomonas sp.シクロヘキシルアミンオキシダ-ゼの補酵素の推定--菌の生育度に及ぼすビタミン拮抗体の阻害作用を利用して
- Comparative Studies on the Mutagenicity of 1,1′-Ethylene-Bis-(1-Nitrosourea) and Related Compounds in Escherichia coli in Relation to the Formation of DNA Interstrand Cross-Links
- オクタデシル硫酸ナトリウムのラットによる代謝
- Comparative Binding Studies on 1,1′-Ethylene-Bis(1-Nitrosourea) and Some 1-Alkyl-1-Nitrosoureas with Nucleic Acids and Proteins In Vitro
- 細菌酵素および肝ミクロゾームとサイトゾルによる AF-2,パラニトロ安息香酸ならびにニューコクシンの還元の比較
- Percutaneous Absorption of α-Olefin Sulfonate (AOS) in Rats
- Biochemical Studies on 1,1'-Ethylenebis (1-nitrosourea) (EBNU). I. Degradation of EBNU in Mild Conditions
- 注射用コカルボキシラ-ゼの安定性
- 細菌および肝ミクロゾームのアゾならびにニトロ還元酵素の比較
- パンクレアチンの脂肪消化作用に及ぼすタ-ル色素の影響について
- 糖尿病患者における赤血球アルドース還元酵素量と糖病合併症との関連
- Characterization of Aldose Reductase and Aldehyde Reductase from the Medulla of Rat Kidney
- Synthesis and Aldose Reductase Inhibitory Activities of Benzyl 2-Oxazolecarbamate Analogues
- Inhibition of Aldose Reductases from Rabbit Lens by Oxazole Derivatives
- Carbethoxylation and pH Profiles of Kinetic Parameters of 20β-Hydroxysteroid Dehydrogenase from Streptomyces hydrogenans
- Interaction of Nicotinamide Adenine Dinucleotide with 20β-Hydroxysteroid Dehydrogenase from Streptomyces hydrogenans
- Structural Requirements in 20-Oxo-steroids for Interaction with the Binding Site of 20β-Hydroxysteroid Dehydrogenase
- Interactions of Sepharose-Bound Neurophysin I and II with Oxytocin and Vasopressin
- Structural Requirements in 20-Oxo-steroids for Interaction with the Catalytic Site of 20β-Hydroxysteroid Dehydrogenase
- Steroid-Protein Interaction : Substrate Specificity of 20β-Hydroxysteroid Dehydrogenase
- Stabilization of 20β-Hydroxysteroid Dehydrogenase by Glycerol
- The Chemical Modifications and Their Effects on the Hormone-Binding Ability of Bovine Neurophysin I
- Photooxidation of Bovine Neurophysin II in the Presence of Rose Bengal
- The Role of Single Tyrosine and Histidine Residues in Bovine Neurophysin I
- 8. テトロースの還元を促進する酵素(ビタミンB研究委員会第200回会議研究発表要旨)
- Metabolism of Triphenylmethane Colors. I. Absorption, Excretion, and Distribution of Guinea Green B (FD and C Green No. 1) in Rats
- The Metabolism of Saccharin and the Related Compounds in Rats and Guinea Pigs
- 生体試料中のシクロヘキシルアミンの螢光定量法
- pHスタット
- Hydrolysis of 4-Methylumbelliferyl Tetra-N-acetyl-β-chitotetraoside by Lysozyme and Its Inhibition by N, N', N"-Triacetylchitotriose
- 家兎水晶体Aldose Reductase IIの基質特異性と種々の化合物による阻害
- Characterization of Aldose Reductases Ia and Ib from Rabbit Lens
- 家兎水晶体のDL-グリセルアルデヒド還元酵素活性の多様性
- Purification and Some Properties of Aldose Reductase from Rabbit Lens
- Sorbitol Pathway in Lenses of Normal Diabetic Rabbits
- 酵素製剤の品質に関する研究-2-市販キモトリプシン及びトリプシン製剤の製剤学的検討
- 酵素製剤の定量試験-1-リゾチ-ム製剤